HIV_2 RNA Quantitation

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General Information

Lab Name
HIV_2 RNA Quantitation
Lab Code
HIV2VL
ORCA Name
HIV 2 RNA Quantitation
Description

The quantitative HIV2VL assay is used to monitor HIV-2 RNA in plasma from known HIV-2-infected patients. This RT-PCR assay targets the Long Terminal Repeat (LTR) region of the HIV-2 genome.

NOTE: This test is temporarily being referred by UW Laboratory Medicine to the New York State Dept of Health Bloodborne Viruses Laboratory (5/10/19). Continue to order the test through UW Lab Medicine. The New York State Dept of Health will not accept samples sent directly to them (unless by prior arrangement).

Synonyms
HIV-2 RNA , HIV-2 viral load
Components

Interpretation

Method

TQPCR

Reference Range
See individual components
Ref. Range Notes

Normal = not detected.

Quantitative range of assay is 10 to 100,000 HIV-2 RNA copies/mL plasma/CSF

Ordering & Collection

Specimen Type
Blood, CSF
Collection

10 mL blood in LAVENDER TOP (EDTA) tube or

Blood in a single 5 or

8.5 mL PEARL TOP (EDTA) PPT tube or

3 - 4 mL of CSF in sterile collection tube

Handling Instructions

Send sample to lab for processing within 6 hours of collection.

Quantity
requested: 3 to 4 mL EDTA plasma or CSF
minimum: 2 mL EDTA plasma or CSF

Processing

Processing

Lavendar Top tube: Centrifuge, aliquot 3 to 4 mL plasma into polypropylene tube within 6 hours of collection and freeze at -70°C or colder.

Pearl Top tube: Centrifuge within 6 hours of collection and freeze at -70 °C or colder.

CSF: Freeze at -70 °C or colder.

Note: Sample should be a dedicated collection tube that has not been accessioned for any other assay, unless approved by Retrovirus Lab.

Performance

LIS Dept Code
Virology, HMC (HVIR)
Performing Location(s)
HMC Virology, Retrovirus
206-897-5210
Other Locations/Notes

NOTE: This test is temporarily being referred to New York State Department of Health-Wadsworth Center, David Axelrod Institute, Bloodborne Viruses Laboratory, 120 New Scotland Avenue, Albany, NY 12208

(5/10/2019)

Frequency
Results available in approximately 3 weeks. (NYSDOH)
Available STAT?
No

Billing & Coding

CPT codes
87539
LOINC
69354-9