HIV_2 RNA Quantitation

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General Information

Lab Name
HIV_2 RNA Quantitation
Lab Code
HIV2VL
ORCA Name
HIV 2 RNA Quantitation
Description

The quantitative HIV2VL assay is used to monitor HIV2 RNA in plasma from known HIV-2 infected patients. This RT-PCR assay targets the Long Terminal Repeat (LTR) region of the HIV-2 genome.

Synonyms
HIV 2 RNA, HIV 2 Viral Load
Components

Interpretation

Method

TQPCR

Reference Range
See individual components
Ref. Range Notes

Normal = not detected.

Quantitative range of assay is 10 to 100,000 HIV2 RNA copies/mL plasma

Ordering & Collection

Specimen Type
Blood
Collection

10 mL blood in LAVENDER TOP (EDTA) tube or blood in an 8.5 mL PEARL TOP (EDTA) PPT tube

Handling Instructions

Send sample to lab for processing within 6 hours of collection.

Quantity
requested: 3 to 4 mL EDTA plasma
minimum: 2 mL EDTA plasma

Processing

Processing

Lavendar Top tube: Centrifuge. Aliquot 3 to 4 mL plasma into polypropylene tube within 6 hours of collection and freeze at -70°C or colder.

Pearl Top tube: Centrifuge within 6 hours of collection and freeze at -70 °C or colder.

Performance

LIS Dept Code
Virology, HMC (HVIR)
Performing Location(s)
HMC Virology, Retrovirus
206-897-5210
Frequency
Once every 2 weeks; results within 3 weeks
Available STAT?
No

Billing & Coding

CPT codes
87539
LOINC
69354-9