Hepatitis B Surface Antibody (HBsAb)

General Information

Lab Name

Hepatitis B Surface Ab

Lab Code

HBSA

Epic Ordering

Hepatitis B Surface Antibody

Description

The Quantitative detection Hepatitis B virus Surface IgG antibody (HBSAb) in human sera using the FDA approved Abbott ARCHITECT AUSAB-DIL test two-step chemiluminescent immunoassay.

In the first step, sample, assay diluent, and recombinant Hepatitis B surface Antigen (rHBsAg) coated paramagnetic microparticles are combined. Anti-HBs present in the sample binds to the rHBsAg coated microparticles. In the second step, rHBsAg acridinium-labeled conjugate is added, which binds to IgG anti-HBs. Then pre-trigger and trigger solutions are added to the reaction mixture. The resulting chemiluminescent reaction is measured as relative light units (RLUs).

A direct relationship exists between the amount of anti-HBs in the sample and the RLUs. The concentration of anti-HBs in the sample is determined using an active ARCHITECT AUSAB calibration curve. Results are reported as mIU/mL.

For Batteries containing HBSAb see:

Hepatitis B Antibodies (HBcAB, HBsAB) [HBAB], Quantitative detection of Hepatitis B virus Surface IgG antibody (HBSAb) and Hepatitis B virus Core IgG and IgM antibodies (HBCAb)

Hepatitis B Battery [HBB], Quantitative detection of Hepatitis B virus Surface IgG antibody (HBSAb), Qualitative detection of Hepatitis B virus Surface Antigen (HBSAg) and Qualitative detection of Hepatitis B virus Core IgG and IgM antibodies (HBCAb)

Hepatitis B Surface Antigen & Antibody (HBsAg, HBsAb) [HBSS], Quantitative detection of Hepatitis B virus Surface IgG antibody (HBSAb) and Qualitative detection of Hepatitis B virus Surface Antigen (HBSAg)

Synonyms

HBsAB-IgG, Hepatitis Titer

Components

Code	Name
BSAB	Hepatitis B Surface Ab
IUAB	Hepatitis B Surf Ab Intl Units
IN7	Hepatitis B Surface Ab Interp

Interpretation

Method: Chemiluminescence Immunoassay
Reference Range: See individual components
Ref. Range Notes: Per manufacture’s package insert protective level is ≥12 mIU/mL.

Ordering & Collection

Specimen Type: Blood
Collection: Blood: 5 mL blood in GOLD TOP serum separator tube (SST).

Also acceptable: 5 mL blood in ORANGE TOP rapid serum tube (RST) or RED TOP tube
Handling Instructions
Quantity: requested: 1 mL serum
minimum: 0.5 mL serum

Processing

Processing: Blood: Specimens may be stored on or off the clot, red blood cells, or separator gel for up to 3 days at room temperature (15-30°C) or up to 7 days at 2-8°C. If testing will be delayed more than 3 days for specimens stored at room temperature or more than 7 days for specimens stored at 2-8°C, remove serum from the clot, red blood cells, or separator gel and store at -20°C or colder. Avoid more than three freeze/thaw cycles.

Outside Laboratories: Specimens may be stored on or off the clot, red blood cells, or separator gel for up to 3 days at room temperature (15-30°C) or up to 7 days at 2-8°C. If testing will be delayed more than 3 days for specimens stored at room temperature or more than 7 days for specimens stored at 2-8°C, remove serum from the clot, red blood cells, or separator gel and store at -20°C or colder. Avoid more than three freeze/thaw cycles.

Unacceptable samples: Cord blood, neonatal specimens, cadaver specimens, heat-inactivated specimens, or body fluids such as saliva, urine, amniotic fluid, or pleural fluid. Specimens with obvious microbial contamination or are grossly hemolyzed.

Performance

LIS Dept Code

Clinical Virology (CVIR)

Performing Location(s)

Other	Virology 206-685-8037 Virology Laboratory, Ste 320 1616 Eastlake Ave E. Seattle, WA 98102

Frequency

Monday-Friday

Available STAT?

Billing & Coding

CPT codes: 86706
LOINC: 10900-9
Interfaced Order Code: UOW110