Hepatitis B Envelope Antigen and Antibody

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General Information

Lab Name
Hepatitis B e Ag and Ab by EIA
Lab Code
HBE
Epic Ordering
Hepatitis B Envelope Antigen and Antibody
Description

The Qualitative detection of Hepatitis B virus “e” antigen (HBeAg) and Hepatitis B virus “e” antibody (HBeAb) in human sera by the FDA approved LIASON® IgG Assay.

The presence of HBeAg in serum is associated with presence of Dane particles and Hepatitis B virus (HBV) DNA polymerase enzyme. These markers suggest active HBV replication. Hepatitis B Surface antigen (HBsAg) positive and HBeAg positive persons are much more likely to transmit infections to others. The development of anti-HBe during acute Hepatitis B infection is a marker of resolution of illness.

Synonyms
Components

Interpretation

Method

The qualitative determination of HBeAg is a direct, sandwich chemiluminescence immunoassay (CLIA). The qualitative determination of anti-HBe is a competitive sandwich chemiluminescence immunoassay (CLIA) based on neutralization.

Reference Range
See individual components
Guidelines

Ordering & Collection

Specimen Type
Blood
Collection

Blood: 5 mL blood in GOLD TOP serum separator tube (SST).

Also acceptable: 5 mL blood in ORANGE TOP rapid serum tube (RST), RED TOP tube or the following anticoagulants EDTA, heparin, or citrate.

Handling Instructions
Quantity
requested: 2 mL serum or plasma
minimum: 0.6 mL serum or plasma

Processing

Processing

Blood: Harvest serum/plasma from whole blood within 24 hour or collection. Serum/plasma may be stored at 2-8°C (ice pack) for up to 3 days at room temperature (15-30°C) or up to 7 days. Freeze at -20°C or colder for long term storage. Do not freeze/thaw.

Outside Laboratories: Ship samples on Dry Ice, do not freeze/thaw. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack), shipment time must not to exceed 7 days from collection. Samples stored at 2-8°C greater than 7 days old will be rejected.

Unacceptable samples: Cord blood, neonatal specimens, cadaver specimens, heat-inactivated specimens, or body fluids such as saliva, urine, amniotic fluid, or pleural fluid. Specimens with obvious microbial contamination, lipemic or are grossly hemolyzed.

Performance

LIS Dept Code
Clinical Virology (CVIR)
Performing Location(s)
Other Virology
206-685-8037

Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102
Frequency
Once per week.
Available STAT?
No

Billing & Coding

CPT codes
86707, 87350
LOINC
18719-5
Interfaced Order Code
UOW1183