Influenza A, B & RSV PCR Panel

General Information

Lab Name

Influenza A/B & RSV by PCR

Lab Code

FLURSV

Epic Ordering

Influenza A/B and RSV by PCR

Description

The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the modified FDA approved Panther Fusion® Flu A/B/RSV assay for the rapid detection and differentiation of human influenza A/B, and respiratory syncytial virus (RSV) using real-time PCR.

The assay targets the influenza A viruses (matrix gene), the influenza B viruses (matrix gene) and RSV (matrix gene). Results are reported as Detected or NOT detected and a semi-qualitative result is provided by reporting the PCR Cycle threshold (Ct) value for positive specimens.

Note: Due to supply chain variables and logistical workflows to minimize turn-around time, orders may be substituted for medically equivalent qualitative assays at an equivalent or cheaper cost at some hospital locations. CPT/PLA codes may differ. Substituted tests may not contain Ct values in all circumstances. Call the laboratory with questions.

Synonyms

Influenza A, Influenza B, Respiratory syncytial virus (RSV)

Components

Code	Name
FLUSD	Influenza A/B & RSV Specimen
FLAPCR	Influenza A PCR Result
CTFLA	Influenza A Ct Value
FLBPCR	Influenza B PCR Result
CTFLB	Influenza B Ct Value
RSVPCR	RSV PCR Result
CTRSV	RSV Ct Value
FLURI	Influenza A/B & RSV Interp

Interpretation

Method: Real-time Polymerase Chain Reaction.
Reference Range: See individual components

Ordering & Collection

Specimen Type: Nasopharyngeal swab, Oropharyngeal swab, Anterior Nares swab, Broncho alveolar lavage (BAL) and tracheal aspirate. NOTE: The FDA approved Simplexa Flu/AB assay is verified for NP swabs only.
Collection: Collect swab specimens according to standard technique using a polyester-, rayon-, or nylon-tipped swab. Immediately place the swab specimen into VTM/UTM. Do not use calcium alginate swabs. Refrigerate (2-8°C) within 4 hours of collection.

Collect Broncho alveolar lavage (BAL) and tracheal aspirates in a sterile container.

Minimum sample requirement: 0.75 mL
Handling Instructions: Transport specimens on ice (ice Pak), store at 2 to 8°C for up to 72 hours post collection. If there is a greater than 72 hours delay before testing of the specimen, freeze at -70°C or colder and ship on dry ice. Do not freeze/thaw.
Quantity: requested: Swab in UTM
minimum: 750 µl

Processing

Processing: Send sample to UW Virology Laboratory within 4 hours of collection. If sample cannot arrive within 4 hours of collection, store sample at 2-8°C (ice pack) for up to 72 hours of collection. If there is a greater than 72 hour delay before testing sample, freeze sample at -70°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Samples stored at 2-8°C greater than 72 hours old may be rejected. Do not freeze/thaw.

Performance

LIS Dept Code

Central Lab Virology (RVIR)

Performing Location(s)

CL	Virology, Renton 206-685-6656 1602 Raymond Ave SW Renton, WA 98057-3356	206-685-6656 opt 4

Frequency

Test is performed multiple times a day; expect same-day results for specimens received in the morning, next-day otherwise.

Available STAT?

Billing & Coding

CPT codes: 87631
LOINC: 81655-3
Interfaced Order Code: UOW2745