CMV Qualitative by PCR, Tissue/Cells

General Information

Lab Name

CMV Qualitative PCR, Tissue/Cells

Lab Code

CMVQLT

Epic Name

CMV Qualitative PCR, Tissue/Cells

Description

The detection of CMV by real-time PCR amplification. PCR detects amplicons of the immediate-early (IE) viral protein 1 (IE1, UL123) and Glycoprotein B (gB, UL55) coding regions. Detection of CMV DNA (IU/mL) is achieved by amplifying a standard curve at the same efficiency, consisting of serial dilutions of a known amount of DNA containing the CMV amplicons. Results are reported as Not Detected or Positive. The minimum virus level that gives a positive result in 95% of replicates is 20 IU/mL (1.3 log IU/mL).

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For Blood, CSF, or Clear non-cellular fluid (e.g. BAL, or Washing) and Swab see CMV Quantitative by PCR [CMVQN].

For Dried Blood Spot, see CMV Quant by PCR, Blood Spots [CMVBSQ].

For Eye Fluid, see CMV Quant PCR for Eye Fluids [CMVEYE] or Eye Fluid Viral PCR Quant Panel [EYEVQP].

Synonyms

Cytomegalovirus by PCR-Tissue or Cells, PCR-CMV-Tissue or Cells

Components

Code	Name
CMVQLS	CMV Qual Specimen Description
CMVQLR	CMV Qual Result
CMVQLI	CMV Qual Interpretation

Interpretation

Method: Real-time Polymerase Chain Reaction.
Reference Range: See individual components

Ordering & Collection

Specimen Type: Tissue Biopsy, Paraffin Tissue, Bone Marrow or Cellular fluid (e.g. Washing)
Collection: Tissue Biopsy: Pea size amount in sterile tube or universal transport media (UTM).

Paraffin Tissue: DO NOT SEND ENTIRE BLOCK and DO NOT SEND SLIDES. Slice 3-4 slices that are 5-50 micron thick in sterile tube.

Bone Marrow: 5 mL blood in EDTA (LAVENDER TOP) tube, No Heparin aspirate in syringe.

Cellular fluid: 1 mL in sterile tube

Unacceptable samples: Buffy coat collected with heparin syringe. Paraffin slides.

Minimum sample requirement: 0.5 mL
Handling Instructions: Bone Marrow: 5 mL bone marrow in EDTA (LAVENDER TOP) tube to Laboratory at room temperature within 24 hour of collection.

Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.
Quantity: Requested: See Collection
Minimum: 0.5 mL

Processing

Receiving Instructions: Tissue Biopsy: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Paraffin Tissue: Ship at room temperature.

Bone Marrow: Ship at room temperature within 24 hours of collection.

Cellular fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Outside Laboratory:

Buffy coat: Separate buffy coat from bone marrow within 24 hours of collection, Freeze at -10°C or colder.

Tissue/Cellular fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Shipment time must not exceed 48 hours from collection if stored at 2-8°C. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Misc Sendout

Performance

Lab Department

Virology, Eastlake(EVIR)

Frequency

Wednesday. Results following Thursday.

Available STAT?

Performing Location(s)

Other	Virology, Molecular 206-685-6656 Virology Laboratory, Ste 320 1616 Eastlake Ave E. Seattle, WA 98102

Billing & Coding

CPT Codes: 87496
LOINC: 4998-1
Interfaced Order Code: UOW722