CMV Quant PCR for Eye Fluids

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General Information

Lab Name
CMV Quant PCR for Eye Fluids
Lab Code
CMVEYE
Epic Ordering
CMV Quantitative PCR, Eye Fluids
Description

The detection and quantitation of CMV by real-time PCR. The PCR detects amplicons of the immediate-early (IE) viral protein 1 (IE1, UL123) and Glycoprotein B (gB, UL55) coding regions. Quantitation of CMV DNA (IU/mL) is achieved by amplifying a standard curve at the same efficiency, consisting of serial dilutions of a known amount of DNA containing the CMV amplicons. Results are reported in International Units per milliliter (IU/mL). The minimum virus level that gives a positive result in 95% of replicates is 300 IU/mL (2.48 log IU/mL).

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For Blood, CSF or Clear non-cellular fluid (e.g. BAL or Washing) and Swab see CMV Quantitative by PCR [CMVQN].

For Tissue Biopsy, Bone Marrow, or Cellular fluid (e.g. Washing), see CMV Qualitative by PCR, Tissue/Cells [CMVQLT].

For Dried Blood Spot, see CMV Quant by PCR, Blood Spots [CMVBSQ].

Synonyms
CMV-Vitreous
Components

Interpretation

Method

Real-time Polymerase Chain Reaction.

Reference Range
See individual components

Ordering & Collection

Specimen Type
Eye Fluid
Collection

Eye Fluid: 50 µL in sterile container.

Unacceptable Samples: Do not send syringe with needle. Only send syringe or transfer fluid to sterile container.

Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.

Handling Instructions
Quantity
requested: 50 microliters
minimum: 50 microliters

Processing

Processing

Eye Fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Outside Laboratories: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Shipment time must not exceed 48 hours from collection if stored at 2-8°C. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Performance

LIS Dept Code
Central Lab Virology (RVIR)
Performing Location(s)
CL Virology, Renton
206-685-6656

1602 Raymond Ave SW

Renton, WA 98057-3356

206-685-6656 opt 4
Frequency
Monday-Saturday. Results same day.
Available STAT?
No

Billing & Coding

CPT codes
87497
LOINC
24041-6
Interfaced Order Code
UOW3052