BK Virus by PCR Quantitative
General Information
- Lab Name
- BK Virus PCR Quant
- Lab Code
- BKVQN
- Epic Ordering
- BK Virus PCR Quant
- Description
Blood (EDTA Plasma)
The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the FDA approved Roche cobas® BKV Quantitative assay for the detection of BK polyomavirus (BKV) using real-time PCR amplification.
The lower limit of detection, LoD (the minimum virus level that gives a positive result in 95% of replicates), and the lower limit of quantitation, LLoQ, are both 21.5 IU/mL.
A conversion factor of 1.1 Log10 IU/mL is equivalent to 1.0 Log10 copies/mL.
The Roche cobas® BKV assay uses primers specific for the BKV VP2 region and the BKV small t-antigen region.
For Tissue Biopsy, Bone Marrow, or Cellular fluid (e.g. BAL or Washing), see BK Virus by PCR Qualitative [BKVQL].
For Urine, see BK Virus by PCR Quantitative, Urine [UBKQN].
- Synonyms
- Polyomavirus Type BK-Quant
- Components
-
Code Name BKVQNS BK Virus Quant Specimen Type BKVQNR BK Virus Quant Result BKLOG BK Virus log IU/mL BKVQNI BK Virus Quant Interpretation
Interpretation
- Method
Real-time Polymerase Chain Reaction
- Reference Range
- See individual components
- Ref. Range Notes
Not Applicable
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
Blood: 3 mL EDTA plasma or PPT
Unacceptable samples: Plasma collected in sodium or lithium heparin.
Note: Separate specimens MUST be submitted when both Serology and PCR testing are ordered.
- Handling Instructions
Freshly drawn whole blood specimens may be held at 2 to 25°C for up to 24 hours prior to plasma preparation through centrifugation.
- Quantity
-
requested: 1.0 mL of EDTA plasma
minimum: 350µL of EDTA plasma
Processing
- Processing
Blood: Centrifuge upon arrival, remove plasma from cells.
Plasma specimens may be stored and/or transported:
- 2 to 30°C for up to 24 hours
- 2 to 8°C for up to 6 days
- -15°C to -80°C for long-term storage up to 6 months.
Outside Laboratory: Ship samples on dry ice. If freezer and/or dry ice is unavailable, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 6 days of collection. Do not freeze/thaw.
Performance
- LIS Dept Code
- Central Lab Virology (RVIR)
- Performing Location(s)
-
CL Virology, Renton
206-685-66561602 Raymond Ave SW
Renton, WA 98057-3356
206-685-6656 opt 4
- Frequency
- Tuesday and Friday. Results same day.
- Available STAT?
- No
Billing & Coding
- CPT codes
- 87799
- LOINC
- 32284-2
- Interfaced Order Code
- UOW274