Anti Neutrophil Cytoplasm Panel
General Information
- Lab Name
- Anti Neutrophil Cytoplasm Panel
- Lab Code
- ANCAP
- Epic Name
- Anti Neutrophil Cytoplasm Panel
- Description
For neutrophil surface antibodies, see information under Lab Mnemonic: 102.
Anti-neutrophil cytoplasmic antibodies are detected by indirect immunofluorescence (IFA) using human neutrophil cellular preparations as substrate. The pattern and titer are reported on samples with positive results. The reported patterns are C-ANCA (cytoplasmic), P-ANCA (perinuclear), and atypical. Antibodies to human neutrophil cytoplasmic antigens (ANCA) are associated with autoimmune vasculitides such as Granulomatosis with Polyangiitis (GPA) and Microscopic Polyangiitis (MPA). The results of this assay should be used in conjunction with patient history, clinical findings and other test results for diagnosis.
- Synonyms
- ANCA-Panel, ANCAG, Atypical ANCA, C ANCA, MPO, P ANCA, PR3, Vasculitis, Wegener's Antigen
- Components
-
Code Name ANCAIF Antineutrophil Cytoplasmic Antibody by IF APR3L Anti PR3 Level APR3C Anti PR3 Comment AMPOL Anti MPO Level AMPOC Anti MPO Comment
Interpretation
- Guidelines
- Method
Indirect immunofluorescence (IFA) using INOVA ethanol fixed and formalin fixed neutrophils to detect autoantibodies.
- Reference Range
- See individual components
- Ref. Range Notes
Established by testing healthy blood donors during method validation.
- Interferences and Limitations
Strong anti-nuclear antibody (ANA) staining may resemble and/or obscure ANCA patterns. Antibodies to cytoskeletal fibers can mimic a C-ANCA pattern.
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
6 mL blood in Red Top or GOLD SST tube
- Handling Instructions
Note: If requested STAT the ordering physician MUST call the Laboratory Medicine Resident on-call for approval.
- Quantity
-
Requested: 2 mL serum
Minimum: 1 mL serum
Processing
- Receiving Instructions
Separate serum and refrigerate. Stable 14 days refrigerated. Stable long term frozen.
- Misc Sendout
Performance
- Lab Department
- Immunology(IMM)
- Frequency
- Monday-Friday
- Available STAT?
- Lab Medicine Resident approval required.
- Performing Location(s)
-
UW-MT Immunology
206-520-4600Clinical Lab, Room NW220,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195
Billing & Coding
- CPT Codes
- 83520x2, 86036
- LOINC
- 18719-5
- Interfaced Order Code
- UOW1551