Adenoviruses, PCR Quantitative

General Information

Lab Name

Adenoviruses, PCR Quant

Lab Code

ADVQN

Epic Name

Adenoviruses Quantitative PCR

Description

The detection and quantitation of Adenovirus (ADV) by real-time PCR amplification. The assay is a multiplex assay divided into 2 reactions. PCR reaction “1” detects amplicons of the hexon region for subgroups A and F. PCR reaction “2” detects amplicons of the hexon region of subgroups B, D, and E and the penton region for subgroup C. Quantitation of ADV DNA (copies/mL) is achieved by amplifying two standard curves consisting of serial dilutions of a known amount of DNA containing the ADV A & F and ADV B, D, E & C amplicons. Results are reported in copies per milliliter (copies/mL) Log 10. The minimum virus level that gives a positive result in 95% of replicates is 300 copies/mL (2.48 log copies/mL).

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For Tissue Biopsy, Bone Marrow, Swab, or Cellular fluid (e.g. BAL or Washing), see Adenoviruses by PCR Qualitative, Tissue or Cells [ADVQLT].

For Urine, see Adenovirus by PCR Quantitative, Urine [UADVQN].

Synonyms

PCR-Adenoviruses

Components

Code	Name
ADVQNS	ADV, Specimen Desc, PCR Quant
ADVQNA	ADV A,F Result, PCR Quant
ADALOG	ADV A,F DNA Copies/mL (Log 10)
ADVQNB	ADV BCDE Result, PCR Quant
ADBLOG	ADV BCDE DNA Copies/mL (Log10)
ADVQNI	ADV Interpretation, PCR Quant

Interpretation

Method: Real-time Polymerase Chain Reaction.
Reference Range: See individual components
Ref. Range Notes: Not Applicable

Ordering & Collection

Specimen Type: Blood, CSF, or Clear non-cellular fluid (e.g. BAL, or Washing)
Collection: Blood: 3 mL blood in EDTA (LAVENDER TOP) tube.

CSF: 1 mL in sterile container.

Clear non-cellular fluid (e.g. BAL or Washing): 1 mL in sterile container.

Also Acceptable: 3 mL blood in GOLD SST, RED, or PPT (PEARL TOP) tube.

Unacceptable samples: Plasma collected in sodium or lithium heparin.

Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.
Quantity: Requested: 1.0 mL
Minimum: 0.5 mL

Processing

Receiving Instructions: Blood: Separate plasma/serum from whole blood within 24 hours of collection. Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

CSF: If CSF has cellular material, centrifuge & aliquot clear CSF. Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Clear non-cellular fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Outside Laboratory: Ship samples on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Misc Sendout

Performance

Lab Department

Virology, Eastlake(EVIR)

Frequency

Monday and Thursday.

Available STAT?

Performing Location(s)

Other	Virology, Molecular 206-685-6656 Virology Laboratory, Ste 320 1616 Eastlake Ave E. Seattle, WA 98102

Billing & Coding

CPT Codes: 87799
LOINC: 49340-3
Interfaced Order Code: UOW684