Yeast (Fungi) MIC Susceptibility Panel

General Information

Lab Name
Yeast MIC Susceptibility Panel
Lab Code
YSTMIC
Epic Name
Yeast MIC Susceptibility Panel
Description

Panel includes the following anti-fungal agents: amphotericin B, micafungin, fluconazole and voriconazole. Other anti-fungal agents are available on request: caspofungin, 5-flucytosine, posaconazole and itraconazole.

Method Information:

The TREK Sensititre YeastOne® susceptibility test panel is a colorimetric micro-dilution susceptibility test to determine quantitative antifungal susceptibilities of Candida sp. Antifungal agents tested are in ranges of concentrations as well as the colorimetric indicator of growth, alamar blue. Panels are innoculated with a standardized suspension of organisms and incubation at 35C for 24 to 48 hours. The minimum inhibitory concentrations (MIC) for the test organism are determined by assessing the lowest antifungal concentration showing inhibition of growth as evidenced by no color change.

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine. It has not been cleared or approved by the US Food and Drug Administration. This test is used for clinical purposes. It should not be regarded as investigational or for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical laboratory testing.

References

Clinical and Laboratory Standards Institute 2012. Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts: Fourth informational supplement [M27-S4].CLSI, Wayne, PA.

Components

Interpretation

Guidelines
Method

Broth Microdilution

Reference Range
See individual components

Ordering & Collection

Specimen Type
Yeast isolate in pure culture
Collection

Send pure culture of Candida species. Susceptibility testing for other non-Candida species isolates and molds will be referred for testing to an outside laboratory.

Quantity
Requested: One plate or slant

Processing

Receiving Instructions

Outside Laboratories: Send pure culture at room temperature or refrigerated. Follow approved regulations for transporting infectious substances.

Misc Sendout

Performance

Lab Department
Microbiology(MC)
Frequency
Daily
Available STAT?
No
Performing Location(s)
UW-MT Microbiology
206-520-4600

Clinical Lab, Room # NW177,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195

Billing & Coding

LOINC
29577-4
Interfaced Order Code
UOW2778