Wound Bacterial Culture and Sensitivity (Special Anaerobic) with Gram Stain

General Information

Lab Name

Wound C/S w/Gram (Anaerobic)

Lab Code

WNDANC

Epic Ordering

Wound C/S w/Gram (Anaerobic)

Description

Specify exact source of specimen.

This culture is intended for abscess, hardware, deep and/or sterile wound sites, or when rule out anaerobes is required.

If rule out Actinomyces is required as well as routine culture, order additional separate culture R/O Actinomyces Bacterial Culture and Sensitivity [ACTINC].

Superficial wounds (skin and superficial sites such as boil, furuncle, cyst, ulcer, superficial abrasion, first degree burn, impetigo, folliculitis, and cellulitis), see Wound Bacterial Culture and Sensitivity with Gram Stain [WNDC].

Synonyms

Abscess Anaerobic Culture, Deep wound, WNDPHC

Components

Reference Range

See individual components

Guidelines

Specimen Type: Abscess, deep wound
Collection: Remove surface exudate before collecting specimen.

Preferred specimen: Abscess fluid in syringe. Remove needle, expel air and cap syringe.

Swab: Preferred method of transport is ESwab. Gel swab is acceptable.

Unacceptable: Specimens collected in Universal Transport Medium (UTM); dry swabs; syringes with needles; specimens collected with expired swabs/transport media.
Handling Instructions: Specify exact source of specimen and "R/O Anaerobes" for optimal workup

Abscess fluid: Transport in syringe (expel air, remove needle and cap syringe), ideally within 2 hours of collection, but not more than 24 hours after collection.

Swabs: Transport at room temperature, ideally within 4 hours of collection, but not more than 24 hours after collection.

Cold temperature reduces the viability of anaerobes.

Specimens will be rejected if received > 24 hours after collection. Viability of organisms is significantly reduced after 24 hours.
Quantity: requested: Eswab or as much fluid as possible
minimum: Eswab or 1 mL fluid

Processing: If specimen is described as "superficial", or if site is not specified and rule out anaerobes is not requested, log in WNDC.

LIS Dept Code

Microbiology (MC)

Performing Location(s)

HMC	Microbiology 206-744-5858 325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420
UW-MT	Microbiology 206-520-4600 Clinical Lab, Room # NW177, University of Washington Medical Center, 1959 NE Pacific street, Seattle, WA 98195

Other Locations/Notes

UW-MT Microbiology performs testing for UW-MT and UW-NW.

Frequency

Processed upon receipt

Available STAT?

Yes-Gram stain