VZV Quant PCR for Eye Fluids

The detection and quantitation of Varicella Zoster Virus (VZV) by real-time PCR amplification. The primers used in this PCR reaction amplify a fragment of the ORF62 region. Quantitation of VZV DNA (copies/mL) is achieved by amplifying a standard curve in each run, consisting of serial dilutions of a known amount of DNA containing the VZV amplicons. The minimum virus level that gives a positive result in 95% of replicates is 75 copies/mL (1.88 log copies/mL). Results are reported in copies per milliliter (copies/mL).

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For Blood or Clear non-cellular fluid (e.g. BAL or Washing) see Varicella Zoster Quantitative by PCR [VZVQN].

For Tissue Biopsy, Bone Marrow, or Cellular fluid (e.g.BAL or Washing), see Varicella Zoster Qualitative by PCR, Tissue or Cells [VZVQLT].

For CSF and Swab see Varicella Zoster Rapid PCR, Swab or CSF [VZVRPD].

Eye Fluid: 50 microliters in sterile container.

Unacceptable Samples: Do not send syringe with needle. Only send syringe or transfer fluid to sterile container.

Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.

Eye Fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Outside Laboratories: Ship samples on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.