VZV Quant PCR for Eye Fluids

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General Information

Lab Name
VZV Quant PCR for Eye Fluids
Lab Code
VZVEYE
Epic Ordering
Varicella zoster Quantitative PCR, Eye Fluid
Description

The detection and quantitation of Varicella Zoster Virus (VZV) by real-time PCR amplification. The primers used in this PCR reaction amplify a fragment of the ORF62 region. Quantitation of VZV DNA (copies/mL) is achieved by amplifying a standard curve in each run, consisting of serial dilutions of a known amount of DNA containing the VZV amplicons. The minimum virus level that gives a positive result in 95% of replicates is 75 copies/mL (1.88 log copies/mL). Results are reported in copies per milliliter (copies/mL).

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For Blood or Clear non-cellular fluid (e.g. BAL or Washing) see Varicella Zoster Quantitative by PCR [VZVQN].

For Tissue Biopsy, Bone Marrow, or Cellular fluid (e.g.BAL or Washing), see Varicella Zoster Qualitative by PCR, Tissue or Cells [VZVQLT].

For CSF and Swab see Varicella Zoster Rapid PCR, Swab or CSF [VZVRPD].

Synonyms
VZV-Vitreous
Components

Interpretation

Method

Real-time Polymerase Chain Reaction.

Reference Range
See individual components

Ordering & Collection

Specimen Type
Eye Fluid
Collection

Eye Fluid: 50 microliters in sterile container.

Unacceptable Samples: Do not send syringe with needle. Only send syringe or transfer fluid to sterile container.

Note: Separate specimens MUST be submitted when both Viral Culture/Serology and PCR testing are ordered.

Handling Instructions
Quantity
requested: 50 microliters
minimum: 50 microliters

Processing

Processing

Eye Fluid: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Outside Laboratories: Ship samples on dry ice. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack). Shipment time must not exceed 48 hours from collection. Sample may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Performance

LIS Dept Code
Virology, Eastlake (EVIR)
Performing Location(s)
Other Virology, Molecular
206-685-6656

Virology Laboratory, Ste 320
1616 Eastlake Ave E.
Seattle, WA 98102
Frequency
Tuesday and Friday. Results same day.
Available STAT?
No

Billing & Coding

CPT codes
87799
LOINC
49451-8
Interfaced Order Code
UOW3054