Von Willebrand Factor Multimer Analysis
General Information
- Lab Name
- VWF Multimer Analysis
- Lab Code
- VWMULT
- Epic Name
- Von Willebrand Factor Multimer Analysis
- Description
von Willebrand Factor (VWF) multimer proteins are separated according to their molecular weight by agarose gel electrophoresis, subjected to immunofixation with anti-VWF antiserum and visualized using peroxidase-labeled antibody.
The electrophoretic patterns of high, intermediate and low molecular weight multimers are analyzed and used in conjunction with other von Willebrand disease (VWD) tests (Factor 8 activity, VWF antigen and activity and collagen binding) to type/subtype VWD. The multimer test is also useful in suspected cases of acquired VWD and when results of other vWD tests (Factor VIII, VWF Antigen and Activity, VWF collagen binding) are abnormally low or discordant.
- Synonyms
- VWF Multimer
- Components
-
Code Name MULTI VWF Multimer Analysis MULTIM VWF Multimer Method
Interpretation
- Method
Agarose gel electrophoresis with immunofixation
- Reference Range
- See individual components
- Ref. Range Notes
High, Intermediate and Low Molecular Weight Multimers Present
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
3 or 5 mL BLUE (CITRATE) tube
- Handling Instructions
The Laboratory MUST process specimen within 4 hrs of blood collection.
- Quantity
- Requested: entire sample
Processing
- Receiving Instructions
UW-MT Instructions: Take specimen to UW-MT Coag lab for processing. Coag tech will freeze plasma sample for transport to HMC Coag.
HMC Instructions: Take specimen to HMC Coag lab for processing.
Outside Laboratory: Centrifuge for 10 minutes, remove plasma & re-spin plasma for another 10 minutes. Decant & Freeze plasma (minimum 1.0 mL) @ -20°C to -80°C. Send Frozen on dry ice.
- Misc Sendout
Performance
- Lab Department
- Coagulation(COAG)
- Frequency
- Run on Wednesday and result entered by end of day Thursday.
- Available STAT?
- No
- Performing Location(s)
-
HMC Coagulation
206-520-4600325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420
Billing & Coding
- CPT Codes
- 85247
- LOINC
- 6013-7
- Interfaced Order Code
- UOW3497