JC Virus by PCR Quantitative, Urine

General Information

Lab Name

JCV PCR Quant, URN

Lab Code

UJCVQN

Epic Ordering

JCV Quantitative PCR, Urine

Description

The detection and quantification of JC Virus (JCV) by real-time PCR amplification. The primers used in this PCR reaction amplify the Large T antigen gene. The JC Virus probes are labeled with reporter dye FAM and quencher TAMRA. Quantification of JC Virus DNA (copies/mL) is achieved by amplifying standard curves consisting of serial dilutions of a known number of DNA containing the JC Virus amplicons. The minimum virus level that gives a positive result in 95% of replicates is 500 copies/mL (2.70 log copies/mL).

This test was developed and its performance characteristics determined by the University of Washington Department of Laboratory Medicine and Pathology. It has not been cleared or approved by the US Food and Drug Administration.

For Blood, CSF, or Clear non-cellular fluid (ex: BAL, or Washing), see JC Virus by PCR Quantitative [JCVQN].

For Tissue Biopsy, Bone Marrow, Swab, or Cellular fluid (ex: BAL or Washing), see JC Virus Qualitative PCR, Tissue [JCVQLT].

Synonyms

JC Virus, PML Virus, Progressive Multifocal Leukoencephalopathy virus

Components

Code	Name
UJCVQ	JC Virus PCR Quant, URN
UJCVLG	JCV DNA Copies/mL (Log10),URN
UJCVQI	JC Virus PCR Interp Quant, URN

Interpretation

Method: Real-time Polymerase Chain Reaction
Reference Range: See individual components

Ordering & Collection

Specimen Type: Urine
Collection: Urine: 10 mL Random or Clean catch urine in sterile container.

Note: Separate specimens MUST be submitted when both Viral Culture or Serology and PCR testing are ordered.

Unacceptable Samples: Urine with Preservative.
Handling Instructions
Quantity: requested: 10 mL Urine
minimum: 5 mL Urine

Processing

Processing: Urine: Send urine at 2-8°C (ice pack) within 48 hours of collection. Sample may be stored at 2-8°C (ice pack) for up to 48 hours from collection. Freeze at -10°C or colder. Do not freeze/thaw.

Outside Laboratories: Ship samples on dry ice, do not freeze/thaw. If freezer and/or dry ice is not available, ship immediately at 2-8°C (ice pack), shipment time must not exceed 48 hours from collection. Samples stored at 2-8°C greater than 48 hours old may be rejected.

Performance

LIS Dept Code

Virology, Eastlake (EVIR)

Performing Location(s)

Other	Virology, Molecular 206-685-6656 Virology Laboratory, Ste 320 1616 Eastlake Ave E. Seattle, WA 98102

Frequency

Tuesday. Results same day.

Available STAT?

Billing & Coding

CPT codes: 87799
LOINC: 49411-2
Interfaced Order Code: UOW1668