Treponema Pallidum DNA detection, Mucocutaneous

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General Information

Lab Name
Treponema Pallidum DNA detection, Mucocutaneous
Lab Code
TPLSWB
Epic Ordering
Treponema pallidum DNA Detection, Mucocutaneous
Description

Detection of Treponema pallidum DNA.

This test detects Treponema pallidum, the etiologic agent of syphilis and endemic treponematoses (e.g., yaws and pinta) in mucocutaneous swabs. It can be ordered as a primary diagnostic when syphilis is suspected.

Please note that this assay does NOT distinguish subspecies of T. pallidum.

http://depts.washington.edu/molmicdx/

Synonyms
23S LAMP, 23S PCR, chancre, darkfield microscopy, FTA-ABS, meningovascular, neurosyphilis, RPR, spirochete, Syphilis, tabes dorsalis, TP-PA, TPPA, Treponema, Treponema pallidum, treponeme, VDRL
Components

Interpretation

Method

DNA extraction, nucleic acid purification, Loop Mediated Isothermal Amplification

Reference Range
See individual components
Guidelines

Ordering & Collection

Specimen Type
UTM Swab
Collection

Preferred specimen: Mucocutaneous swabs, including genital, oral, anal/rectal and lesions

Acceptable specimen: Mucocutaneous swabs, including genital, oral, anal/rectal and lesions

Unacceptable specimen:

  • Citrated or heparinized solutions
  • Tissues floating in formalin
  • Swab/fluid collected in tube containing agar
Handling Instructions

Please see Molecular Microbiology Specimen Submission for complete specimen collection and handling instructions.

Quantity
requested: 650uL
minimum: 500uL

Processing

Processing

UWMC/HMC: Store and send UTM swabs refrigerated or frozen. If specimen storage and transport will exceed 48 hours, freeze at -20 to -80°C. SPOT to route to first would be Virology Renton Annex

Performance

LIS Dept Code
Micro Molecular Diag (MMD)
Performing Location(s)
UW-MT Microbiology, Molecular Diagnostics
206-520-4600

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Shipping Address

Attn: Molecular Microbiology
UW CLSPS
1601 Lind Ave SW Room 117
Renton, WA 98057
Phone: 206-520-4600
Alternate phone: 206-598-6147

Performing Lab Address

Clinical Microbiology Lab, NW177
University of Washington Medical Center
1959 NE Pacific Street
Seattle, WA 98195
Phone: 206-598-5735
Alternate phone: 206-598-6147

Contact Information

Please e-mail us with any questions or comments you may have. Your inquiry will be answered as soon as possible.

email: molmicdx@uw.edu

The Molecular Microbiology lab is open from Monday-Friday, 7am-4pm PDT.

Billing inquiries and requests for faxed reports can be made to our Client Services Department at (206) 520-4600 or (800) 713-5198.

For results or other inquiries, we can be reached by phone at the following numbers:

  • Phone: (206) 598-5735
  • Alternate phone: (206) 598-6147
  • FAX: (206) 520-4903

For assistance during weekends, holidays and after hours, please contact Lab Medicine Resident at (206) 598-6190
Frequency
Mondays and Thursdays. Batched and currently performed 1 – 2 times per week.
Available STAT?
No

Billing & Coding

CPT codes
87798
LOINC
41163-7
Interfaced Order Code
UOW5585