Tacrolimus by LC-MS/MS
General Information
- Lab Name
- Tacrolimus by LCMSMS
- Lab Code
- TACROG
- Epic Name
- Tacrolimus Level
- Description
The concentration of tacrolimus in whole blood is determined by liquid chromatography-tandem mass spectrometry after protein precipitation. The reportable range is 1.0 to 195.0 ng/mL with imprecision between 4 and 7 %CV.
- Synonyms
- F506, FK506, PROGRAF, TACRO
- Components
-
Code Name TACRO Tacrolimus by LCMSMS TACINF Tacrolimus Information
Interpretation
- Method
Whole blood protein precipitation. Analysis by liquid chromatography - tandem mass spectrometry.
- Reference Range
- See individual components
- Ref. Range Notes
A broad consensus therapeutic range for 12-hour trough tacrolimus concentrations is 5-20 ng/mL. Other target ranges may be specified by various protocols, depending on type of transplant, time since transplant, concomitant immunosuppressive therapy and sampling time.
Ordering & Collection
- Specimen Type
- whole blood
- Collection
-
3 mL blood in a LAVENDER TOP tube. Trough specimen preferred.
- Quantity
-
Requested: entire sample
Minimum: 1.5 mL whole blood for 3 mL Lavender Liquid EDTA (K3 EDTA); 0.5 mL whole blood for 3 mL Lavender Dry EDTA (K2 EDTA)
Processing
- Receiving Instructions
Refrigerate whole blood, do not spin Frozen sample acceptable - Do not repeat freeze-thaw cycle
Outside Laboratories: transport specimen at 2-8°C
Stability: Room Temp: 2 d; Refrig: 5 d
- Misc Sendout
Performance
- Lab Department
- Chemistry, University(CHU)
- Frequency
- Daily, specimens in UW Chemistry lab by 1:00pm results by 4:30pm
- Available STAT?
- No
- Performing Location(s)
-
UW-MT Chemistry, Special
206-520-4600Clinical Lab, Room NW220,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195
Billing & Coding
- CPT Codes
- 80197
- LOINC
- 74097-7
- Interfaced Order Code
- UOW2752