Porphyrins Quantitative, Random Urine (Sendout)

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General Information

Lab Name
Porphyrins Quantitative, Random Urine
Lab Code
RUPORR
Epic Name
Porphyrins, Quant, Random Urine (Sendout)
External Test Id
PQNRU
Description

Useful For: Assessment for congenital erythropoietic porphyria and porphyria cutanea tarda and during symptomatic periods for acute intermittent porphyria, hereditary coproporphyria, and variegate porphyria in random urine specimens.

    Patient Preparation: Patient should abstain from alcohol for 24 hours prior to collection. Include a list of medications the patient is currently taking.

      Ordering Note: This test is for random urine specimens only. For testing on timed specimens, refer to separate entry Porphyrins Quantitative, 24 Hour Urine (Sendout) [RUPORG].

        Collection Note: Limited specimen stability. Collect Monday through Thursday only to ensure arrival and testing at Mayo Clinic Laboratories within 72 hours of collection.

          ***Note: Effective 12/02/2025, this test will be reported under Reference Laboratory Test 1 (SEND1) due to reference range, unit, and component changes at Mayo. Regular reporting will resume on January 5th, 2026 when LIS battery changes are complete.***

          Synonyms
          Acute Intermittent Porphyria (AIP), Congenital Erythropoietic Porphyria (CEP), Coproporphyrin, Hereditary Coproporphyria (HCP), PBG (Porphobilinogen), Porphyria Cutanea Tarda (PCT), PQNRU, T596, Uroporphyrin, Variegate Porphyria (VP)
          Components
          Code Name
          RUUPPR Uroporphyrin (Octa), Random Urine
          RUHPPR Heptacarboxylporphyrins, Random Urine
          RUHXPR Hexacarboxylporphyrins, Random Urine
          RUPCPR Pentacarboxylporphyrins, Random Urine
          RUCPPR Coproporphyrin (tetra), Random Urine
          RUPRBR Porphobilinogen, Random Urine
          RUPRIR Porphyrins Quantitative Interp, Random Urine

          Interpretation

          Method

          High-Performance Liquid Chromatography (HPLC) with Fluorometric Detection

          • Includes quantitation of coproporphyrins, uroporphyrins, and intermediate porphyrins (heptacarboxyl, hexacarboxyl, and pentacarboxyl).
          • Includes liquid chromatography-tandem mass spectrometry (LC-MS/MS) determination of porphobilinogen.
          Reference Range
          See individual components
          Ref. Range Notes

          Interpretation: Abnormal results are reported with a detailed interpretation which may include an overview of the results and their significance, a correlation to available clinical information provided with the specimen, differential diagnosis, and recommendations for additional testing when indicated and available.

          Interferences and Limitations

          Cautions: Porphobilinogen (PBG) and porphyrins are susceptible to degradation at high temperature, at pH below 5.0, and exposure to light.

          Neither erythropoietic protoporphyria nor X-linked dominant protoporphyria are detected utilizing urine porphyrins and PBG measurements.

          Ethanol and a variety of medications are known to interfere with heme synthesis leading to elevations in urine porphyrins, particularly coproporphyrin. Coproporphyrin elevation without concomitant PBG elevation should not be used as the basis for the diagnosis of porphyria but may warrant follow-up testing with fecal porphyrin analysis.

          Ordering & Collection

          Specimen Type
          Urine
          Collection

          Collect a random urine sample in a sterile container and protect from light. Expedite transport to the lab.

          • Collect samples Monday - Thursday only to ensure arrival and testing at Mayo within 72 hours of collection.
          • Include a list of the patient's current medications.
          Handling Instructions

          Outside Laboratories: A timed collection is recommended due to limited sample stability. Random urine specimens must arrive at Mayo Clinic Laboratories within 72 hours of collection.

          Quantity
          Requested: 20-50 mL urine in light-protected aliquot
          Minimum: 15 mL urine in light-protected aliquot

          Processing

          Receiving Instructions

          Aliquot urine into a light-protected (amber) screw-top container.

          • Also Accepted: Aliquot containers wrapped in foil (ensure primary container is properly labeled).

          Sendouts:

          • Order Mayo Test: PQNRU.
          • Interfaced: Yes.
          • If provided, list the patient's medications in the "Performing Lab Comments" section of the MayoAccess order.
          • Ensure that the sample is protected from light.
          • Samples must arrive at Mayo within 72 hours of collection.

          Stability: Frozen: 72 hours; Refrigerated: Unacceptable; Ambient: Unacceptable.

          Misc Sendout

          Performance

          Lab Department
          Sendouts Mayo Lab (FZ)(MAFZ)
          Frequency
          Performed: Monday - Friday. Report Available: 2-4 days from sample receipt.
          Available STAT?
          No
          Performing Location(s)
          Sendout Mayo Clinic Laboratories
          800-533-1710

          200 First Street Southwest
          Rochester, MN 55901

          Billing & Coding

          CPT Codes
          84110, 84120
          LOINC
          30896-5
          Interfaced Order Code
          UOW5770