5 Hydroxyindolacetic Acid Level, Urine (Sendout)

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General Information

Lab Name
5_Hydroxyindolacetic Acid, URN
Lab Code
RUHIA
Epic Ordering
5-Hydroxyindolacetic Acid Level, Urine (Sendout)
External Test Id
HIAA
Description

Useful for: Biochemical diagnosis and monitoring of intestinal carcinoid syndrome.

Patient Preparation:

  1. Some medications could interfere with test results. The ordering provider should decide if any medications should be stopped and when they should be restarted. If clinically feasible, discontinue the following medications at least 48 hours prior to, as well as during, specimen collection: Acetaminophen (Tylenol or generic versions), Aspirin, Cough syrups, Cold and flu medications.
  2. For 48 hours prior to, as well as during, the urine collection, the patient should limit the following to 1 serving per day: Fruits, vegetables, nuts, caffeinated beverages or foods.

Note: If a random urine specimen (or timed collection <22 hours or >26 hours) is submitted, the 5-Hydroxyindoleacetic Acid, Random Urine test will be performed and results will be reported under Reference Laboratory Test 1 (SEND1).

Synonyms
5 HIAA-Urine, 5-HIAA (5-Hydroxyindolacetic Acid), 5-OH-Indoleacetic Acid, Carcinoid Syndrome, HIAA, Hydroxyindoleacetic Acid, Serotonin Metabolite, Serotonin-Urine
Components

Interpretation

Method

Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Reference Range
See individual components

Ordering & Collection

Specimen Type
Urine
Collection

24-Hour Urine:

  • Add 25 mL of 50% acetic acid as preservative in collection container at the start of collection (preferred).
    • For children <5 years old, use 15 mL of 50% Acetic Acid.
  • Refer to Mayo Urine Preservatives-Collection and Transportation for 24-Hour Urine Specimens for additional information on acceptable collection conditions.
  • Collect urine for 24 hours. Refrigerate within 4 hours of completion of collection.
  • The collection interval must be between 22 and 26 hours. Samples with collection intervals <22 hours and >26 hours will be rejected by Mayo for the 24-hour test, but can be processed for the random test with approval from the provider.
  • The addition of preservative or application of temperature controls must occur within 4 hours of completion of the collection.
Handling Instructions

Outside Laboratories:

  • Mix specimen well. Measure and record total volume. Note the collection interval and total volume on the requisition.
  • Aliquot urine into a screw-capped vial and refrigerate.
  • The collection interval must be between 22 and 26 hours. Samples with collection intervals <22 hours and >26 hours will be rejected by Mayo for the 24-hour test, but can be processed for the random test.
Quantity
requested: 10 mL urine from a 24-hour urine collection
minimum: 1 mL urine from a 24-hour urine collection

Processing

Processing

SPS:

  • Mix urine and record volume. Note the collection interval and total volume on the requisition.
  • Transfer 10 mL urine to a plastic, screw-capped vial.
  • Refrigerate sample.

Result-at-Entry:

  • RHIATV (5_HIAA Total Volume, URN): <Enter total volume in mL>
  • RHIINT (5_HIAA Interval, URN): <Enter collection interval in hours>

Sendouts:

  • Order Mayo Test: HIAA.
  • Interfaced: Yes.
  • For random urine collections, or collections with intervals <22 hrs or >26 hrs, order Mayo Test: HIAAR (5-HIAA, Random Urine).

Stability: Refrigerated (preferred): 56 days; Frozen: 1 year; Ambient: Unacceptable.

Performance

LIS Dept Code
Sendouts Mayo Lab (RF) (MARF)
Performing Location(s)
Sendout Mayo Clinic Laboratories (Superior Drive)
800-533-1710

3050 Superior Drive NW
Rochester, MN 55901
Frequency
Performed: Monday - Friday. Report Available: 3-5 days.
Available STAT?
No

Billing & Coding

CPT codes
83497
LOINC
1695-6
Interfaced Order Code
UOW3012