Eosinophils, Urine (Sendout)

Interpretation

Method

Hansel Stain

Reference Range

See individual components

Ref. Range Notes

Reference Value: 0%

Interpretation: Results of greater than 5% eosinophils are indicative of acute interstitial nephritis; results between 1% and 5% eosinophils are indeterminant.

Ordering & Collection

Specimen Type

Urine

Collection

Collect 10 mL random urine in a sterile, leak-proof container.

• Clean catch, mid-stream samples are preferred to minimize contamination and improve accuracy.
• Collect Monday - Friday only. Expedite transport to the lab. Samples must be processed within 2 hours of collection.

Handling Instructions

Outside Laboratories: Prepare unstained slides within 2 hours of collection. Random urine specimens will not be accepted.

Processing Instructions:

1. Collect a random urine specimen.
2. Centrifuge 10 mL of a random urine collection.
3. Pour off supernatant. Assess urine sediment for presence of white blood cells (WBC). If no WBCs are seen, the eosinophil test can't be performed. If WBCs are present, prepare slides using the centrifuged sediment.
4. Unstained slide should be prepared within 2 hours of collection.
5. Centrifuge urine in cytospin centrifuge at speed of 750 rpm for 5 minutes.
6. Guidelines for slide preparation. Use the sediment to prepare slides as follows:
   
   • 150 mcL of sample for 1 to 3 WBC/high power field (hpf)
   • 100 mcL of sample for moderate to normal sediment (<50 cell/hpf)
   • 50 mcL of sample for heavier sediment (>50 cells/hpf)
   • 25 mcL for packed fields of sediment
7. Label slides with patient's full name and date of birth.

Stability:

• Unpreserved Urine: Ambient: 2 hours; Refrigerated: Unacceptable; Frozen: Unacceptable.
• Prepared Slides: Ambient (preferred): 14 days; Refrigerated: 14 days; Frozen: 14 days.

Quantity

Requested: 10 mL Urine
Minimum: 5 mL Urine

Processing

Receiving Instructions

Forward sample to Hematology for processing as soon as possible.

Hematology:

1. Centrifuge 10 mL of a random urine collection.
2. Pour off supernatant. Assess urine sediment for presence of white blood cells (WBC). If no WBCs are seen, the eosinophil test can't be performed. If WBCs are present, prepare slides using the centrifuged sediment.
3. Unstained slide should be prepared within 2 hours of collection.
4. Centrifuge urine in cytospin centrifuge at speed of 750 rpm for 5 minutes.
5. Guidelines for slide preparation. Use the sediment to prepare slides as follows:
   • 150 mcL of sample for 1 to 3 WBC/high power field (hpf)
   • 100 mcL of sample for moderate to normal sediment (<50 cell/hpf)
   • 50 mcL of sample for heavier sediment (>50 cells/hpf)
   • 25 mcL for packed fields of sediment
6. Trim the smaller CID label (RUEOSN "Slide Label") at the indicated line and affix it to the slide, folding it to the underside to prevent any overhang without covering the unfrosted portion of the slide. Label the slide carrier with the larger CID label that includes the patient's full name and date of birth. Reprint label as needed.
7. Insert the prepared slide into the slide carrier and forward to Sendouts.

Sendouts:

• Order Mayo Test: EOSU1
• Interfaced: Yes [Interface: 601; Worksheet: MAAB]
• Ensure that slides are labeled with the patients full name and date of birth (required by Mayo).

Stability:

• Unpreserved Urine: Ambient: 2 hours; Refrigerated: Unacceptable; Frozen: Unacceptable.
• Prepared Slides: Ambient (preferred): 14 days; Refrigerated: 14 days; Frozen: 14 days.

Misc Sendout

Performance

Lab Department

Sendouts Mayo Lab (AB)(MAAB)

Frequency

Performed: Monday - Sunday. Report Available: 1 day.

Available STAT?

No

Performing Location(s)

Sendout	Mayo Clinic Laboratories 800-533-1710 200 First Street Southwest Rochester, MN 55901

Billing & Coding

CPT Codes

85999

LOINC

32014-3

Interfaced Order Code

UOW4979