2,3 Dinor 11 Beta Prostaglandin F2 Alpha, 24h Urine (Sendout)

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General Information

Lab Name
2,3 Dinor 11 Beta Prostaglandin F2 Alpha, 24h URN (Sendout)
Lab Code
RU23BP
Epic Name
2,3-Dinor 11 Beta-Prostaglandin F2 Alpha, 24 Hour Urine (Sendout)
External Test Id
23BPT
Description

Useful For: Screening for mast cell activation disorders including systemic mastocytosis. 2,3-Dinor-11beta-prostaglandin F2 alpha (2,3 BPG) is elevated in the urine of patients with systemic mastocytosis (SM). When 2,3 BPG is used in combination with Leukotriene E4, 24 Hour Urine (Sendout) [RULTE4] and N Methylhistamine, 24 Hour Urine (Sendout) [RUNMHT], the sensitivity for SM detection increases to 90%.

    Elevated levels of 2,3-dinor-11beta-prostaglandin F2 alpha (2,3 BPG) in urine are not specific for systemic mast cell disease and may be found in patients with angioedema, diffuse urticaria, or myeloproliferative diseases in the absence of diffuse mast cell proliferation.

      Systemic mast cell disease is a heterogeneous disease, and some patients may not have elevated 2,3 BPG in urine.

        Patient Preparation: Patients taking aspirin or nonsteroidal anti-inflammatory drugs (NSAID) may have decreased concentrations of prostaglandin F2 alpha. If possible, discontinue for 2 weeks or 72 hours, respectively, prior to collecting a specimen.

          Ordering Requirements: Test pre-approved for orders originating from Fred Hutch, UW Medicine, or The Vancouver Clinic Allergy Clinics (MSALL, UESALL, VANTVC). Orders from other locations require Laboratory Medicine Resident (LMR) approval.

            Ordering Note: This test is for timed urine collections only. For random urine collections, refer to 2,3-Dinor 11 Beta-Prostaglandin F2 Alpha, Random Urine (Sendout) [RUR23B].

            Synonyms
            11 Beta-Prostaglandin F2 Alpha, 11BPG, 2 3 11 Beta-Prostaglandin F2 Alpha, 23BPG, 23BPT, BPG2, BPT23, Mastocytosis, Prostaglandin
            Components
            Code Name
            RUMINT Collection Interval
            RUMTV Total Volume
            RU23BR 2,3 Dinor 11 Beta Prostaglandin F2 Alpha, 24 Hour Urine
            RUCRE Creatinine, 24 Hour Urine
            RUCREC Creatinine Concentration, 24 Hour Urine

            Interpretation

            Method

            2,3-Dinor-11 Beta-Prostaglandin F2 Alpha: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

            Creatinine: Enzymatic Colorimetric Assay

            Reference Range
            See individual components
            Ref. Range Notes

            Reference Range:

            2,3 BPG: <1802 pg/mg creatinine

            Interpretation: Elevated urine 2,3-dinor-11beta-prostaglandin F2 alpha is consistent with systemic mastocytosis.

            Ordering & Collection

            Specimen Type
            Urine
            Collection

            Collection Note: A 24-hour urine collection is the preferred specimen type, but a random specimen is also acceptable.

            24 Hour Collection: Collect urine for 24 hours in a preservative-free amber polyethylene urine collection container. Refrigerate during collection (preferred). Record the collection interval.

            • The application of temperature controls (refrigeration) must occur within 4 hours of completion of the collection.
            • If the total volume provided is less than 300 mL, the order will be changed to the random urine test by Mayo Clinic.

            Approval Required
            Test pre-approved for orders originating from Fred Hutch, UW Medicine, or The Vancouver Clinic Allergy Clinics (MSALL, UESALL, VANTVC). Orders from all other locations require Laboratory Medicine Resident (LMR) approval.
            Handling Instructions

            Outside Laboratories:

            • Mix urine well. Measure and record total volume. Document the collection interval and total volume on the DLMP requisition.
            • Aliquot 5 mL urine to a plastic screw-cap vial and refrigerate specimen.

            Stability: Refrigerated (Preferred): 14 days; Frozen: 30 days; Ambient: 8 hours.

            Quantity
            Requested: 5 mL urine
            Minimum: 4 mL urine

            Processing

            Receiving Instructions
            • The application of temperature controls must occur within 4 hours of completion of the collection.
            • Mix specimen well. Measure and record total volume.
            • Document the collection interval and total volume on the requisition. Provide Sendouts with a copy.
            • Aliquot 5 mL urine to a plastic, screw-capped vial and refrigerate.

            Result-at-entry:

            • RUMINT (Collection Interval): <Enter the collection interval in hr.>
            • RUMTV (Total Volume): <Enter the total volume in mL.>

            Sendouts:

            • Order Mayo Test: 23BPT.
            • Interfaced: Yes.

            Stability: Refrigerated (Preferred): 14 days; Frozen: 30 days; Ambient: 8 hours.

            Misc Sendout

            Performance

            Lab Department
            Sendouts Mayo Lab (RF)(MARF)
            Frequency
            Performed: Tuesday, Thursday. Report Available: 3-8 days.
            Available STAT?
            No
            Performing Location(s)
            Sendout Mayo Clinic Laboratories
            800-533-1710

            200 First Street Southwest
            Rochester, MN 55901
            Sendout Mayo Clinic Laboratories (Superior Drive)
            800-533-1710

            3050 Superior Drive NW
            Rochester, MN 55901

            Billing & Coding

            CPT Codes
            84150
            LOINC
            94381-1
            Interfaced Order Code
            UOW5664