2,3-Dinor-11Beta-Prostaglandin F2 Alpha, Random Urine (Sendout)

General Information

Lab Name
Lab Code: 692
External Test Id: 23BPR
Description: Useful For: Screening for mast cell activation disorders including systemic mastocytosis. 2,3-Dinor-11beta-prostaglandin F2 alpha (2,3 BPG) is elevated in the urine of patients with systemic mastocytosis (SM). When 2,3 BPG is used in combination with urinary leukotriene E4 (OLTG #1070) and N-methyl histamine (OLTG #478), the sensitivity for SM detection increases to 90%.

Elevated levels of 2,3-dinor-11beta-prostaglandin F2 alpha (2,3 BPG) in urine are not specific for systemic mast cell disease and may be found in patients with angioedema, diffuse urticaria, or myeloproliferative diseases in the absence of diffuse mast cell proliferation.

Systemic mast cell disease is a heterogeneous disease, and some patients may not have elevated 2,3 BPG in urine.

Patient Preparation: Patients taking aspirin or nonsteroidal anti-inflammatory drugs (NSAID) may have decreased concentrations of prostaglandin F2 alpha. If possible, discontinue for 2 weeks or 72 hours, respectively, prior to collecting a specimen.

Ordering Requirements: Pre-approved for UW Medicine Allergy Clinics. Orders from other locations require Laboratory Medicine Resident (LMR) approval.

Ordering Note: This test is for random urine collections only. For timed collections, refer to 2,3 Dinor 11 Beta Prostaglandin F2 Alpha, 24h Urine (Sendout) [RU23BP].
Synonyms: 11 Beta-Prostaglandin F2 Alpha, 11BPG, 2 3 11 Beta-Prostaglandin F2 Alpha, 23BPG, 23BPR, BPG2, Mastocytosis, Prostaglandin

Interpretation

Method

2,3-Dinor-11 Beta-Prostaglandin F2 Alpha: Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)

Creatinine: Enzymatic Colorimetric Assay

Ref. Range Notes

Reference Range:

2,3 BPG:

<1802 pg/mg creatinine

Interpretation: Elevated urine 2,3-dinor-11beta-prostaglandin F2 alpha is consistent with systemic mastocytosis.

Ordering & Collection

Specimen Type

Urine

Collection

Random Urine Collection: Collect 5 mL random urine in a preservative-free collection container.

Approval Required

Pre-approved for UW Medicine Allergy Clinics. Orders from all other locations require Laboratory Medicine Resident (LMR) approval.

Handling Instructions

Outside Laboratories:

Aliquot 5 mL urine to a plastic screw-cap vial and refrigerate specimen.
Indicate "random collection" on the DLMP requisition.

Stability: Refrigerated (Preferred): 14 days; Frozen: 30 days; Ambient: 8 hours.

Quantity

requested: 5 mL Urine
minimum: 4 mL Urine

Processing

Processing

Random: Transfer 5 mL urine to a plastic, screw-capped vial and refrigerate.

Login: SEND1-;REFRIG

RSNDT1: MAYO
RSTYP1: URN-RAND
RTSRQ1: ;2,3 Dinor 11 Beta Prostaglandin F2 Alpha, Random (Mayo Test 23BPR)

Sendouts:

Order Mayo Test: 23BPR.

Stability: Refrigerated (Preferred): 14 days; Frozen: 30 days; Ambient: 8 hours.

Performance

LIS Dept Code

Performing Location(s)