T SPOT TB Test (Sendout)

General Information

Lab Name

Lab Code

RTBTSP

Epic Name

Not orderable in Epic Order using Lab Undefined [LABUND]

External Test Id

37737

Description

Clinical Significance: The T-SPOT® TB test is an in vitro diagnostic test for the detection of effector T cells that respond to stimulation by Mycobacterium tuberculosis antigens ESAT-6 and CFP 10 by capturing interferon gamma (IFN-g) in the vicinity of T cells in human whole blood. It is intended for use as an aid in the diagnosis of M.tuberculosis infection. The T-SPOT® TB test is an indirect test for M. tuberculosis infection (including disease) and is intended for use in conjunction with risk assessment, radiography and other medical and diagnostic evaluations.

Ordering Guidance: T-SPOT® TB is offered as an alternative Interferon Gamma Release Assay (IGRA) blood test for immunosuppressed patients with indeterminate Quantiferon TB Gold Plus [QFTB4] results. T-SPOT® is NOT a 1st line test.

Collection Note: This is a short stability test. Time blood collections accordingly. Failure to follow collection guidelines may result in test cancellations.

References

QuestDiagnostics T-SPOT®.TB Test FAQ

Synonyms

37737, GRA test for Mycobacterium tuberculosis infection, Interferon-gamma release assay (IGRA) for TB infection, T Spot TB

Components

Code	Name
RTBTSR	T SPOT TB Result
RTBTSI	T SPOT TB Interpretation
RTBTSC	T SPOT TB Comment
RTBSCA	T SPOT TB Panel A Spot Count
RTBSCB	T SPOT TB Panel B Spot Count
RTBTNC	T SPOT TB Negative Control
RTBTPC	T SPOT TB Positive Control
RTBTPL	T SPOT TB Performing Lab

Interpretation

Method

Enzyme-Linked Immunosorbent (ELISPOT) Assay

Reference Range

See individual components

Ref. Range Notes

Reference Range: Negative

References

QuestDiagnostics T-SPOT®.TB Test FAQ

Ordering & Collection

Specimen Type

Blood

Collection

Collect samples Monday through Friday only and expedite transport to the lab.

Samples must arrive in the lab at UW-MT or HMC by 2 p.m. for same-day shipment.
Do not collect the day before a holiday.

Draw: 2 x 10 mL GREEN TOP (Lithium or Sodium Heparin) tubes. Invert tubes to ensure samples are thoroughly mixed.

Unacceptable: Lavender top (EDTA) tube

Handling Instructions

Outside Laboratories: Due to the short sample stability window, outside laboratories must arrange for testing directly with Quest Diagnostics.

UW Medicine Collection Sites: Expedite transport to the laboratory. Samples must arrive in the lab by 2 p.m. for same-day shipment.

Quantity

Requested: 12 mL whole blood
Minimum: 6 mL whole blood (Non-Immunosuppressed patients)

Processing

Receiving Instructions

Hold whole blood at Room Temperature (19-25°C) in the ambient Sendouts rack. Do not refrigerate or freeze. Expedite Processing - Notify sendouts staff upon receipt of samples in lab at UW or HMC.

Sendouts:

Order electronically via QuestQuanum eLabs Account: Quest Diagnostics Nichols SJC.
Order Quest Test: 37737.
Send samples at ambient temperature Monday-Friday on the same day of collection via Quest courier using designated Quest Diagnostics TSPOT specimen bags. (Do not use regular Quest specimen bags, as this will cause the samples to be delayed past stability.)
- UW-MT: Daily courier pickup Monday - Friday.
- HMC: Call for courier pickup.
Additional TSPOT supplies may be obtained by contacting Quest Memphis at: tspot@questdiagnostics.com or (877) 598-2522.

Stability: Ambient: 39 hours (collection to test setup); Refrigerated: Unacceptable; Frozen: Unacceptable.

Reject Due To: Hemolysis, anticoagulants other than lithium or sodium heparin, clotted, centrifuged, samples received past stability.

Misc Sendout

Performance

Lab Department

Sendouts(SO)

Frequency

Collect Monday-Friday only before 1:30pm (in lab by 2). Not available weekends or holidays. Test Turnaround: 7 days.

Available STAT?

Performing Location(s)

Sendout	Quest Diagnostics Nichols Institute 800-642-4657 33608 Ortega Highway San Juan Capistrano, CA 92675

Billing & Coding

CPT Codes: 86481
LOINC: 74281-7