T SPOT TB Test (Sendout)

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General Information

Lab Name
T SPOT TB Test (Sendout)
Lab Code
RTBTSP
Epic Name
T-SPOT TB Test (Sendout)
External Test Id
37737
Description

Clinical Significance: The T-SPOT® TB test is an in vitro diagnostic test for the detection of effector T cells that respond to stimulation by Mycobacterium tuberculosis antigens ESAT-6 and CFP 10 by capturing interferon gamma (IFN-g) in the vicinity of T cells in human whole blood. It is intended for use as an aid in the diagnosis of M.tuberculosis infection. The T-SPOT® TB test is an indirect test for M. tuberculosis infection (including disease) and is intended for use in conjunction with risk assessment, radiography and other medical and diagnostic evaluations.

    Ordering Guidance: T-SPOT® TB is offered as an alternative Interferon Gamma Release Assay (IGRA) blood test for immunosuppressed patients with indeterminate Quantiferon TB Gold Plus [QFTB4] results. T-SPOT® is NOT a 1st line test.

      Collection Note: This is a short stability test. Time blood collections accordingly. Failure to follow collection guidelines may result in test cancellations.

      References
      • QuestDiagnostics T-SPOT®.TB Test FAQ
      Synonyms
      37737, GRA test for Mycobacterium tuberculosis infection, Interferon-gamma release assay (IGRA) for TB infection, T Spot TB
      Components
      Code Name
      RTBTSR T SPOT TB Result
      RTBTSI T SPOT TB Interpretation
      RTBTSC T SPOT TB Comment
      RTBSCA T SPOT TB Panel A Spot Count
      RTBSCB T SPOT TB Panel B Spot Count
      RTBTNC T SPOT TB Negative Control
      RTBTPC T SPOT TB Positive Control
      RTBTPL T SPOT TB Performing Lab

      Interpretation

      Method

      Enzyme-Linked Immunosorbent (ELISPOT) Assay

      Reference Range
      See individual components
      Ref. Range Notes

      Reference Range: Negative

      References
      • QuestDiagnostics T-SPOT®.TB Test FAQ

      Ordering & Collection

      Specimen Type
      Blood
      Collection

      Collect samples Monday through Friday only and expedite transport to the lab.

      • Samples must arrive in the lab at UW-MT or HMC by 2 p.m. for same-day shipment.
      • Do not collect the day before a holiday.

      Draw: 2 x 10 mL GREEN TOP (Lithium or Sodium Heparin) tubes. Invert tubes to ensure samples are thoroughly mixed.

      Unacceptable: Lavender top (EDTA) tube

      Handling Instructions

      Outside Laboratories: Due to the short sample stability window, outside laboratories must arrange for testing directly with Quest Diagnostics.

      UW Medicine Collection Sites: Expedite transport to the laboratory. Samples must arrive in the lab by 2 p.m. for same-day shipment.

      Quantity
      Requested: 12 mL whole blood
      Minimum: 6 mL whole blood (Non-Immunosuppressed patients)

      Processing

      Receiving Instructions

      Hold whole blood at Room Temperature (19-25°C) in the ambient Sendouts rack. Do not refrigerate or freeze. Expedite Processing - Notify sendouts staff upon receipt of samples in lab at UW or HMC.

      Sendouts:

      • Order electronically via QuestQuanum eLabs Account: Quest Diagnostics Nichols SJC.
      • Order Quest Test: 37737.
      • Send samples at ambient temperature Monday-Friday on the same day of collection via Quest courier using designated Quest Diagnostics TSPOT specimen bags. (Do not use regular Quest specimen bags, as this will cause the samples to be delayed past stability.)
        • UW-MT: Daily courier pickup Monday - Friday.
        • HMC: Call for courier pickup.
      • Additional TSPOT supplies may be obtained by contacting Quest Memphis at: tspot@questdiagnostics.com or (877) 598-2522.

      Stability: Ambient: 39 hours (collection to test setup); Refrigerated: Unacceptable; Frozen: Unacceptable.

      Reject Due To: Hemolysis, anticoagulants other than lithium or sodium heparin, clotted, centrifuged, samples received past stability.

      Misc Sendout

      Performance

      Lab Department
      Sendouts(SO)
      Frequency
      Collect Monday-Friday only before 1:30pm (in lab by 2). Not available weekends or holidays. Test Turnaround: 7 days.
      Available STAT?
      No
      Performing Location(s)
      Sendout Quest Diagnostics Nichols Institute
      800-642-4657

      33608 Ortega Highway
      San Juan Capistrano, CA 92675

      Billing & Coding

      CPT Codes
      86481
      LOINC
      74281-7
      Interfaced Order Code
      UOW5883