SC5b-9 Level (Sendout)

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General Information

Lab Name
SC5b 9 Level (Sendout)
Lab Code
RSC5BP
Epic Name
SC5b-9 Level (Sendout)
External Test Id
LAB3458, CWA0200
Description

Test Information: The Soluble Terminal Complement Complex enzyme immunoassay (EIA) measures the amount of the SC5b-9 complex present in human plasma.

Urgent Testing: Testing is performed at Seattle Children's Hospital Laboratory twice weekly, Monday and Thursday. Samples in lab by 09:00 a.m. on Mon/Thurs will be resulted same-day (by 18:00). Otherwise, the turnaround time is 4 days from sample receipt. If expedited testing is required, please contact the Lab Med Resident On-Call to arrange. Expedited testing can be performed Mon-Fri with 24-hour advance notice.

Synonyms
CWA0200, Eculizumab Monitor, LAB3458, MAC, Membrane Attack Complex, SC5b-9, SC5b9, sMAC, Soluble Terminal Complement Complex
Components
Code Name
RC5B9L SC5b 9 Level (Sendout)
RC5B9R SC5b 9 Level

Interpretation

Method

EIA

Reference Range
See individual components
Ref. Range Notes
Reference Range: ≤250 ng/mL

Note: Testing moved from Cincinnati Children's Hospital Lab to Seattle Children's Hospital Lab on 02/02/2026. The test performed by Cincinnati Children's has a reference range of ≤213 ng/mL.

Ordering & Collection

Specimen Type
Blood
Collection

6 mL blood in LAVENDER TOP (EDTA) tube

Handling Instructions

Outside Laboratories: Centrifuge sample, aliquot plasma into a separate plastic vial, and freeze at -20°C within two hours of collection. Transport on dry ice.

Stability: Frozen -20°C (preferred): 6 months; Refrigerated: 24 hours; Ambient: 8 hours.

Unacceptable Conditions: Incorrect anticoagulant, hemolyzed, clotted, or older than 2 hours unspun.

Quantity
Requested: 0.5 mL EDTA plasma + 0.5 mL EDTA plasma for UW431
Minimum: 0.2 mL EDTA plasma

Processing

Receiving Instructions

Centrifuge sample, aliquot plasma into two separate plastic vials, and freeze within 2 hours of collection. If sample volume is insufficient, prioritize the Sendout test.

  • RC5B9L (Sendout Battery): Store in the Sendouts -20°C freezer rack.
  • UW431 (ImmStudy SC5b-9 Level): Store in the Immunology -70°C freezer rack.

Sendouts:

  • Order SEACHL Test: LAB3458
  • Interfaced: Yes [Interface: 601; Department: SOSC]
  • Send samples on dry ice with a copy of the MayoAccess Batch Sheet via DLMP or DE courier.
  • Urgent Sample Handling: In select cases, providers may arrange with SEACHL to perform testing on an unscheduled day. If approved, the LMR will contact SPS/Sendouts asking lab staff to prioritize transport.
    • Write "STAT" on the outgoing MayoAccess Batch Sheet to ensure urgent processing at intake.
    • Urgent samples should be sent immediately via Delivery Express to ensure that they reach Children's before the cutoff for the designated run.

Stability: Frozen -20°C (preferred): 6 months; Refrigerated: 24 hours; Ambient: 8 hours.

Unacceptable Conditions: Incorrect anticoagulant, hemolyzed, clotted, or older than 2 hours unspun.

Misc Sendout

Performance

Lab Department
Frequency
Performed: Monday, Thursday. Turnaround Time: 4 days. Samples in lab by 09:00 Monday or Thursday resulted same-day by 18:00.
Available STAT?
Yes, contact the Laboratory Medicine Resident on-call to arrange for expedited testing.
Performing Location(s)
Sendout Seattle Children's Hospital Laboratories
206-987-2617

4800 Sand Point Way NE
Seattle, WA 98105

Room Number: FB.2.441

Billing & Coding

CPT Codes
86160
LOINC
93244-2
Interfaced Order Code
UOW4025