RBC Genotyping for MNS (Sendout)

Test Information: This test uses PCR to determine or confirm a patient's red cell antigen typing. It does not determine zygosity. In some situations, the genetic typing may not reproduce the red cell phenoytyping results. Test Coverage: M/N; S/s/U (including P2 & NY silencing mutations).

Ordering Guidance: This group of tests can be useful to resolve cases where the patient has inconsistent serological typing or to confirm variants such U variant or U negative.

Ordering Requirements:

• A completed Bloodworks Northwest Genomics Testing Laboratory Requisition must accompany all orders. Leave the billing section blank to be completed by lab staff.

Whole Blood: 10 mL whole blood in LAVENDER TOP (EDTA) tube

• Note: For very difficult draws, contact the Genomics Testing Lab at Bloodworks NW at 206-689-6269 for approval of microtainers.

Cultured Amniocytes: 2 T25 Flasks cultured to confluency.

• Amniotic fluid is not accepted.
• An EDTA whole blood sample from the mother of the fetus is required for cultured amniocyte orders.
• Samples sent on Fridays must be received at Bloodworks Northwest Genomics Lab by 1 p.m.

Labeling Requirements:

• The sample label and requisition must contain two matching identifiers, one being the full name, and the second a numeric identifier like hospital ID/MRN.
• A matching draw date must be included on the specimen label and the requisition form.

Outside Laboratories:

• Store and transport whole blood at ambient temperature.
• Expedite transport; blood samples must be received by Bloodworks NW within 96 hours of collection (48 hours preferred). Allow for one day of transport from UW-MT to Bloodworks Northwest.
  
  • Unless the cells are cultured at UW-MT Cytogenetics and Genomics Lab, clients should arrange to send cultured amniocyte samples directly to Bloodworks NW due to the short stability window (24 hours).
• Submit a completed Bloodworks Northwest Genomics Testing Laboratory Requisition along with the DLMP Requisition.** Leave the billing section blank to be completed by Sendouts staff.
  • **Use client-specific requisition when available. To obtain a client-specific requisition, contact Client Support Services via email at: commserv@uw.edu.

Stability:

• Cultured Amniocytes: Ambient: 24 hours
• Whole Blood: Ambient: 96 hours (48 hours preferred)

*Contact the Bloodworks NW Genomics Lab at (206) 689-6269 for sample acceptability questions prior to cancelling orders outside of the stated stability window.

Store and transport whole blood or cultured amniocytes at ambient temperature.

• Cultured amniocytes should be sent out same-day; notify Sendout staff upon sample receipt. On Fridays, cultured cells must be received at Bloodworks Northwest by the 1 p.m. cutoff.

Sendouts (UW-MT/HMC) and FHCC SPS:

• Bloodworks NW Test: 3117-03
• A completed Bloodworks Northwest Genomics Testing Laboratory Requisition must accompany all orders.
  • The sample label and requisition must contain two matching identifiers, one being the full name, and the second a numeric identifier like hospital ID/MRN.
  • A matching draw date must be included on the specimen label and the requisition form.
• UW-MT/HMC Only: If the institution is not already indicated on the requisition, attach a CAST "Bill/Results To" label.
• Package sample(s) with the associated requisition in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biozard bag will be rejected by Bloodworks NW.
• Send samples via scheduled DLMP Courier pickups or Delivery Express according to the site’s preferred transportation method.
• Cultured amniocytes must be sent same-day. If sent on a Friday, samples must be in lab by 1 p.m.
• Ambient or refrigerated whole blood samples must arrive at Bloodworks within 96 hours of collection (48 hours is preferred).

Stability:

• Cultured Amniocytes: Ambient: 24 hours
• Whole Blood: Ambient: 96 hours (48 hours is preferred)

*Contact the Bloodworks NW Genomics Lab at (206) 689-6269 for sample acceptability questions prior to cancelling orders outside of the stated stability window.