Lymphocyte Subset Analysis (Sendout)

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General Information

Lab Name
Lymphocyte Subset Analysis (Sendout)
Lab Code
RLYMSA
Epic Name
Lymphocyte Subset Analysis (Sendout)
External Test Id
LAB3271
Description

Test Information: This group of assays are used for enumerating the percentages and absolute cell counts for lymphocyte subsets in whole blood. The tests are used in the evaluation of immune function, in the setting of primary and secondary immune deficiencies, post-transplant, and monitoring monoclonal antibody therapies. Multiple panels are available; refer to the Seattle Children's Hospital test menu for further detail: Lymphocyte Subset Analysis.

    Ordering Requirements:

      • Providers must select a specific Lymphocyte Subset Panel when placing the Epic order. Failure to provide this information to the lab may result in significant delays and/or cancellations.
      • Test pre-approved for orders originating from Fred Hutch, UW Medicine, or The Vancouver Clinic Allergy Clinics (MSALL, UESALL, VANTVC). Laboratory Medicine Resident approval is required for all other ordering locations.

      Collection Note: Limited specimen stability. Time blood collections accordingly to ensure that samples reach the performing lab within the 30 hour stability limit.

      Forms & Requisitions
      Synonyms
      ALPS Screen, CD45 RA/RO, LAB3271, Lymphocyte Subsets (Seattle Childrens), T and B Cell Workup, TCR
      Components
      Code Name
      RLYMST Lymphocyte Subset Panel Requested
      RLYMAA Additional Antibodies Requested
      RLYMSR Lymphocyte Subset Analysis Result
      RLYMSL Lymphocyte Subset Analysis Performing Lab

      Interpretation

      Method

      Flow Cytometry

      Reference Range
      See individual components
      Ref. Range Notes

      An intepretive report will be provided.

      Ordering & Collection

      Specimen Type
      Blood
      Collection

      3 mL blood in LAVENDER TOP (EDTA) Tube

      • Also Accepted: Dark Green/Sodium Heparin also acceptable if CBC and differential results are submitted. Microtainers not recommended.

      Collection Timing:

      • Draw Monday through Friday. Do not collect samples on Saturdays or before holidays. If drawn on Sunday, collect after 12 noon.
      • Friday collections must arrive in lab by 13:30 to ensure same-day transport to Seattle Children's.

      Expedite transport to the laboratory. Testing must be initiated by Seattle Children's lab within 30 hours of collection.

      Forms & Requisitions
      Approval Required
      Test pre-approved for orders originating from Fred Hutch, UW Medicine, or The Vancouver Clinic Allergy Clinics (MSALL, UESALL, VANTVC).
      Handling Instructions

      Vancouver Clinic (TVC) Only:

      • Expedite transport to the UW Central Lab to ensure that the samples arrive at Seattle Children's lab within the 30-hour stability.
      • Specify the Lymphocyte Subset Panel and Additional Antibodies requested (if applicable) on the DLMP requisition. Failure to provide this information may result in delays or cancellations.

        Other Outside Laboratories: Due to the limited sample stability for this test, clients should arrange for testing directly with Seattle Children's Hospital Laboratory.

          Stability: Ambient (Required): 30 hours; Refrigerated: Unacceptable; Frozen: Unacceptable.

            Reject Due To: Clotted specimen, Microtainer, Refrigerated.

            Quantity
            Requested: 3 mL whole blood
            Minimum: 1 mL whole blood

            Processing

            Receiving Instructions

            SPS: Short stability; expedite processing. Notify Sendouts staff upon receipt of samples in the lab. Store samples in the ambient Sendouts rack at each site (USENDA/HSENDA/CSENDA).

              Result-at-Entry:

              • RLYMST (Lymphocyte Subset Panel Requested test): <Result will file automatically for orders placed in Epic. For others, enter the panel requested as a free text comment.>
              • RLYMAA (Additional Antibodies Requested): <Result will file automatically for orders placed in Epic. For others, enter the additional antibodies, if requested, as a free text comment.>

              Sendouts (UW-MT or HMC)/Central Lab (RCLSPS):

              • Order SEACHL Test: LAB3271
              • Review the Epic order or TVC manifest to confirm which panel (and additional antibodies, if applicable) is being requested. Complete the SEACHL Cell Marker Requisition accordingly.
              • Expedite transport. Send samples as received via scheduled DLMP Courier pickups or Delivery Express according to the site’s preferred transportation method.
                • AM Collections: Send out same-day.
                • PM Collections: May be held at room temperature for sendout the following morning, unless collected on a Friday.
              • Samples drawn on Friday must be received at SCH same-day.
              • Notify SCH Cell Marker Lab at 206-987-2560 if sending a sample nearing the stability limit or Friday afternoon cutoff.

              Stability: Ambient: 30 hours; Refrigerated: Unacceptable; Frozen: Unacceptable.

              Reject Due To: Clotted specimen, Microtainer, Refrigerated.

              Misc Sendout

              Performance

              Lab Department
              Sendouts(SO)
              Frequency
              Performed: Monday through Friday, Saturday (07:30 to 12:00). Reported: 2-3 days from sample receipt at Seattle Children's Hospital Lab.
              Available STAT?
              No
              Performing Location(s)
              Sendout Seattle Children's Hospital Laboratories
              206-987-2617

              4800 Sand Point Way NE
              Seattle, WA 98105

              Room Number: FB.2.441

              Billing & Coding

              LOINC
              30896-5
              Interfaced Order Code
              UOW6056
              Billing Comments

              CPT codes vary based on panel and additional antibodies requested.