Lymphocyte Crossmatch, STAT (Sendout)

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General Information

Lab Name
Lymphocyte Crossmatch, STAT (Sendout)
Lab Code
RLXS
Epic Name
(not orderable in Epic)
External Test Id
3088-02
Description

Indications: Solid Organ Transplant.

    Test Information: Patient serum is crossmatched against donor lymphocytes to detect antibodies against the donor, and against the patient’s own lymphocytes to detect autoantibodies. STAT orders have a 4- to 8-hour turnaround time with verbal result reporting.

      Ordering Requirements:

      • Providers must notify Bloodworks Northwest HLA Laboratory of any STAT requests by calling (206) 817-7730. If this is not done, the lab will perform the test as routine.
      • Contact information is required for verbal result reporting. Include the name and phone/pager of the person receiving results in the Epic order.
      References
      Forms & Requisitions
      Synonyms
      3088-02
      Components
      Code Name
      RLXAUT Autologous T Cell Crossmatch
      RLXAUB Autologous B Cell Crossmatch
      RLXDNT Donor T Cell Crossmatch
      RLXDNB Donor B Cell Crossmatch
      RLXDID Donor ID

      Interpretation

      Method

      Flow Cytometry technique

      Reference Range
      See individual components
      References

      Ordering & Collection

      Specimen Type
      Blood
      Collection

      3 x 10 mL ACD (YELLOW TOP) tubes and 2 x 10 mL RED TOP tubes

      • Also Accepted: 3 x 10 mL Yellow ACD tubes and 2 x 6 mL RED TOP tubes
      Forms & Requisitions
      Handling Instructions

      Outside Laboratories:

      • Store and transport whole blood at ambient temperature. Expedite transport to UW.
      • A completed Bloodworks Northwest Immunogenetics/HLA Requisition must accompany all orders. In the Billing section, indicate "Bill to UWMC DLMP."

      Stability: Ambient. Samples should be received at Bloodworks Northwest within 72 hours of collection.

      Quantity
      Requested: 30 mL whole blood in Yellow ACD and 20 mL blood Red Top (clot) tubes
      Minimum: 30 mL whole blood in Yellow ACD and 10 mL blood Red Top (clot) tube

      Processing

      Receiving Instructions

      Store and transport samples at ambient temperature.

        Sendouts (UW-MT/HMC) and FHCC SPS:

        • Bloodworks NW HLA Test: 3088-02
        • Interfaced: Yes [Interface: 603; Worksheet: UBWHLA/HBWHLA/FBWHLA or other location-specific worksheet group]
        • Send sample(s) with a copy of the Bloodworks Northwest HLA Requisition or the EPIC requisition (must indicate the test or panel to be ordered at Bloodworks NW).
          • Package sample(s) with the associated requisition in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biohazard bag will be rejected by Bloodworks NW.
        • Send samples via scheduled DLMP Courier pickups or Delivery Express according to the site’s preferred transportation method.
        • STAT orders should be sent to Bloodworks Northwest as received. Depending on the courier schedule, it may be necessary to individually batch a STAT order and/or call Delivery Express for pickup.

        Stability: Ambient. Samples should be received at Bloodworks Northwest within 72 hours of collection.

        Misc Sendout

        Performance

        Lab Department
        Sendouts BWNW Lab (AB)(BWAB)
        Frequency
        Performed: As needed. Results available: 4 to 8 hours from sample receipt at Bloodworks Northwest HLA Lab.
        Available STAT?
        Yes
        Performing Location(s)
        Sendout Bloodworks Northwest Immunogenetics/HLA Laboratory
        206-689-6580

        921 Terry Ave
        6th Floor
        Seattle, WA 98104

        Laboratory staffed for questions: Monday - Friday, 07:00 a.m. to 05:00 p.m.
        After-Hours, Holidays, Weekends: Contact the Lead Technical Coordinator at (206) 817-7730
        Fax: 206-689-6582

        Billing & Coding

        CPT Codes
        86825, 86826
        LOINC
        96046-8