Lymphocyte Crossmatch, Routine (Sendout)

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General Information

Lab Name
Lymphocyte Crossmatch, Routine (Sendout)
Lab Code
RLX
Epic Name
(not orderable in Epic)
External Test Id
3088-00
Description

Indications: Solid Organ Transplant.

    Test Information: Patient serum is crossmatched against donor lymphocytes to detect antibodies against the donor, and against the patient’s own lymphocytes to detect autoantibodies.

    References
    Forms & Requisitions
    Synonyms
    3088-00
    Components
    Code Name
    RLXAUT Autologous T Cell Crossmatch
    RLXAUB Autologous B Cell Crossmatch
    RLXDNT Donor T Cell Crossmatch
    RLXDNB Donor B Cell Crossmatch
    RLXDID Donor ID

    Interpretation

    Method

    Flow Cytometry technique

    Reference Range
    See individual components
    References

    Ordering & Collection

    Specimen Type
    Blood
    Collection

    3 x 10 mL ACD (YELLOW TOP) tubes and 2 x 10 mL RED TOP tubes

    • Also Accepted: 3 x 10 mL Yellow ACD tubes and 2 x 6 mL RED TOP tubes
    Forms & Requisitions
    Handling Instructions

    Outside Laboratories:

    • Store and transport whole blood samples at ambient temperature. Expedite transport to UW-MT.
    • A completed Bloodworks Northwest Immunogenetics/HLA Requisition must accompany all orders. In the Billing section, indicate "Bill to UWMC DLMP."

    Stability: Ambient. Samples should be received at Bloodworks Northwest within 72 hours of collection.

    Quantity
    Requested: 30 mL whole blood in Yellow ACD and 20 mL blood Red Top (clot) tubes
    Minimum: 30 mL whole blood in Yellow ACD and 10 mL blood Red Top (clot) tube

    Processing

    Receiving Instructions

    Store and transport samples at ambient temperature.

      Sendouts (UW-MT/HMC) and FHCC SPS:

      • Bloodworks NW HLA Test: 3088-00
      • Interfaced: Yes [Interface: 603; Worksheet: UBWHLA/HBWHLA/FBWHLA or other location-specific worksheet group]
      • Send sample(s) with a copy of the Bloodworks Northwest HLA Requisition or the EPIC requisition (must indicate the test or panel to be ordered at Bloodworks NW).
        • Package sample(s) with the associated requisition in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biohazard bag will be rejected by Bloodworks NW.
      • Send routine samples via scheduled DLMP Courier pickups or Delivery Express according to the site’s preferred transportation method.

      Stability: Ambient. Samples should be received at Bloodworks Northwest within 72 hours of collection.

      Misc Sendout

      Performance

      Lab Department
      Sendouts BWNW Lab (AB)(BWAB)
      Frequency
      Performed: As needed. Results available: 3 days from sample receipt at Bloodworks Northwest HLA Lab.
      Available STAT?
      No. Select test Lymphocyte Crossmatch, STAT (Sendout) [RLXS] if STAT testing is required.
      Performing Location(s)
      Sendout Bloodworks Northwest Immunogenetics/HLA Laboratory
      206-689-6580

      921 Terry Ave
      6th Floor
      Seattle, WA 98104

      Laboratory staffed for questions: Monday - Friday, 07:00 a.m. to 05:00 p.m.
      After-Hours, Holidays, Weekends: Contact the Lead Technical Coordinator at (206) 817-7730
      Fax: 206-689-6582

      Billing & Coding

      CPT Codes
      86825, 86826
      LOINC
      96046-8