Anti Interferon Gamma Antibody (Sendout)
General Information
- Lab Name
- Anti Interferon Gamma Antibody (Sendout)
- Lab Code
- RIFNGA
- Epic Name
- (not orderable in Epic)
- External Test Id
- IFNGE
- Description
Test Use: Increased susceptibility to BCG or nontuberculous mycobacteria (NTM) may be caused by genetic defects that affect the IL-12 and IFN-gamma pathways. These cytokines are critical to control mycobacterial infections, and disruption of these pathways may lead to severe infections in otherwise healthy individuals. Antibodies to IFN-gamma have been found to result in an acquired form of immunodeficiency that presents as chronic, often extra-pulmonary, infection with NTM.¹ Confirmation of the presence of these autoantibodies may assist with treatment decisions regarding the use of immunomodulation in addition to standard anti-mycobacterial therapy.
Test Algorithm: Anti-IFN-gamma autoantibodies are detected by an initial screening ELISA. If the result is positive, the test reflexes to a flow cytometry assay to determine the ability of the autoantibody to neutralize IFN-gamma by the analysis of IFN-gamma induced phosphorylation of STAT1, a signaling molecule downstream of the IFN-gamma receptor.
Ordering Information: Non-orderable battery included in Anti Interferon Gamma Antibody (Sendout) [RIFNG] package.
- References
- Browne SK, Burbelo PD, Chetchotisakd P, et al. Adult-onset immunodeficiency in Thailand and Taiwan. N Engl J Med. 2012 Aug 23; 367(8): 725-34.
- Forms & Requisitions
- NJH Advanced Diagnostic Labs: Cytokines Diagnostics Requisition
- Synonyms
- Anti-IFNg Autoantibody, Anti-Interferon Gamma antibody, Autoantibody to interferon-gamma, Functional Autoantibodies to cytokines, IFNG, IFNGE, Interferon Gamma Autoantibodies, STAT1 phosphorylation
- Components
-
Code Name RIFNGR Anti Interferon Gamma Antibody ELISA RIFNGI Anti Interferon Gamma Antibody Interpretation RIFNGC Anti Interferon Gamma Antibody Comment
Interpretation
- Method
Enzyme-linked Immunosorbent Assay (ELISA)
Reflex confirmation, if performed: Flow Cytometry
- Reference Range
- See individual components
- References
- Browne SK, Burbelo PD, Chetchotisakd P, et al. Adult-onset immunodeficiency in Thailand and Taiwan. N Engl J Med. 2012 Aug 23; 367(8): 725-34.
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
6 mL blood in GOLD SST or RED TOP tube
- Forms & Requisitions
- NJH Advanced Diagnostic Labs: Cytokines Diagnostics Requisition
- Quantity
-
Requested: 1 mL serum + 1 mL serum for UW436
Minimum: 0.5 mL serum
Processing
- Receiving Instructions
Allow sample to clot at room temperature for 20 to 60 minutes. Centrifuge specimen and transfer 1 mL serum each into two separate plastic vials. If sample volume is insufficient, prioritize the sendout test.
- RIFNGA: Store in -70°C/Superfreeze Sendout rack 93.
- UW436: Store in -70°C/Superfreeze Immunology rack 44.
Sendouts:
- Order NJH Test: IFNGE
- On the NJH Complement/Cytokines requisition under category 7, "Anti-Cytokine Autoantibodies," select IFNGE/Autoantibodies to interferon gamma.
- Ship Monday through Thursday only via FedEx Priority Overnight. Include sufficient dry ice to keep the sample frozen until arrival.
- Do not ship on Fridays.
Stability: Frozen (-70°C): 1 month; Refrigerated: 48 hours; Ambient: Unacceptable.
Unacceptable Conditions: Clotted or hemolyzed samples, plasma.
- Misc Sendout
Performance
- Lab Department
- Sendouts(SO)
- Frequency
- Performed: Monday through Friday. TAT: 14 days.
- Available STAT?
- No
- Performing Location(s)
-
Sendout National Jewish Health Advanced Diagnostic Laboratories
800-550-62271400 Jackson Street
Denver, CO 80206
Billing & Coding
- CPT Codes
- 83516
- LOINC
- 30896-5
- Billing Comments
Reflex flow cytometry testing may be performed at additional cost.