Indirect Antiglobulin (Sendout)

General Information

Lab Name

Lab Code

RIAT

Epic Name

Indirect Antiglobulin (Sendout)

External Test Id

3104-00

Description

Useful For: This test detects the presence of unexpected antibody (allo- or auto-) against red blood cell antigens.

Test Information: Patient serum/plasma is tested against a three cell panel that contains 18 common red cell antigens. The majority of clinically significant red blood cell alloantibodies are detected by this test. If positive, a limited cell panel (antibody identification) will be performed to determine red blood cell antigen specificity. If unresolved, referred to IRL (Immunohematology Reference Laboratory).

Ordering Note: Order this test for sendouts to Bloodworks Northwest only. For testing performed by UW-MT or HMC TSL, refer to Antibody Screen [ABSCR].

Forms & Requisitions

Bloodworks Northwest Request for Blood and Transfusion Testing

Synonyms

3104-00, Antibody Screen, Coombs-Indirect, IAT, Indirect Antiglobulin

Components

Code	Name
RIATR	Indir. Antiglobulin Rslt (Sendout)
S2IAT	Indirect Antiglobulin Testing Lab

Interpretation

Method: Capture solid phase technology or gel column
Reference Range: See individual components

Ordering & Collection

Specimen Type

Blood

Collection

Preferred: 6 mL blood in PINK TOP (EDTA) tube

Also Accepted: 6 mL blood in LAVENDER TOP (EDTA) tube

Pediatric Draws: 1 full 3 mL PINK or LAVENDER (EDTA) tube; Neonate: 2 full 0.5 mL EDTA microtainers (PINK or LAVENDER)

Unacceptable: SST or GEL tubes

Dual Signatures Required: All Blood Bank samples require dual signatures (person collecting the specimen/performing positive patient ID a second
verifier) on the Bloodworks Northwest request form and on the sample label(s).

Forms & Requisitions

Bloodworks Northwest Request for Blood and Transfusion Testing

Quantity

Requested: 6 mL EDTA whole blood
Minimum: 6 mL EDTA whole blood. Pediatric: 1 full 3 mL EDTA; Neonate: 2 full 0.5 ml EDTA microtainers

Processing

Receiving Instructions

Store and transport whole blood at ambient temperature.

Package sample(s) with the associated Bloodworks request form in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biozard bag will be rejected by Bloodworks NW.
Send sample(s) as received via DLMP or Delivery Express courier.

Misc Sendout

Performance

Lab Department

Transfusion Support(TS)

Frequency

Performed: Monday through Sunday. Turn Around Time (from sample receipt at Bloodworks NW): 24 hours on weekdays, 48 hours on weekends. Additional time may be required for positive antibody screens.

Available STAT?

Yes

Performing Location(s)

Sendout	Bloodworks Northwest Central TSL/Immunohematology Reference Laboratory 921 Terry Ave A-Level Seattle, WA 98104	Central Transfusion Support Lab (TSL): 206-689-6525 Immunohematology Reference Lab (IRL): 206-689-6534

Billing & Coding

CPT Codes: 86850
LOINC: 50959-6
Interfaced Order Code: UOW1803