HLA Antibody Specificity (Sendout)

General Information

Lab Name
HLA Antibody Specificity (Sendout)
Lab Code
RHLAS
Epic Name
HLA Antibody Specificity (Sendout)
External Test Id
3083-16C
Description

Indications: Solid Organ Transplant.

    Test Information: To identify and quantify HLA Antibody specificities. It can be used for potential donor selection and/or antibody reduction therapies.

    References
    Synonyms
    3083-16, Calculated Percent Reactive Antibody, CPRA, HLA Antibody Identification
    Components

    Interpretation

    Method

    Flow Cytometry using microparticles (“beads”) coated with recombinant or soluble HLA antigens

    Reference Range
    See individual components

    Ordering & Collection

    Specimen Type
    Blood
    Collection

    10 mL blood in RED TOP (clot) tube

    Also Accepted: 6 mL RED TOP (clot) tube

    Forms & Requisitions
    Handling Instructions

    Outside Laboratories:

    • Store and transport whole blood at ambient temperature (serum from a red top tube is also accepted). Samples must be received by Bloodworks Northwest within 10 days of collection.
    • A completed Bloodworks Northwest Immunogenetics/HLA Requisition must accompany all orders. In the Billing section, indicate "Bill to UWMC DLMP."

    Stability: Ambient: 10 days.

    Quantity
    Requested: 10 mL whole blood in a red top tube
    Minimum: 2 mL whole blood in a red top tube

    Processing

    Receiving Instructions

    Store and transport samples at ambient temperature.

    Sendouts:

    • Bloodworks NW HLA Test: 3083-16
    • Interfaced: Yes [Interface: 603; Worksheet: UBWHLA/HBWHLA/FBWHLA or other location-specific worksheet group]
    • Send sample(s) with a copy of the Bloodworks Northwest HLA Requisition or the EPIC requisition (must indicate the test or panel to be ordered at Bloodworks NW).
      • Package sample(s) with the associated requisition in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biohazard bag will be rejected by Bloodworks NW.
    • Send routine samples via scheduled DLMP Courier shipments or Delivery Express, as needed.
    • STAT orders should be transported to Bloodworks Northwest as received.* Depending on the courier schedule, it may be necessary to individually batch a STAT order and/or call Delivery Express for pickup.
      • *STAT orders for this test are rare and require the provider to request approval from the HLA Lab. If approval is obtained, the HLA Lead Technical Coordinator will contact CSS to request expedited transport of a sample. CSS will create an HC1 task for SPS. If this is not done, orders should be considered routine.

    Stability: Ambient: 10 days. Serum is also accepted.

    Misc Sendout

    Performance

    Lab Department
    Sendouts BWNW Lab (AB)(BWAB)
    Frequency
    Performed: Performed: Monday through Friday. Reported: 5-7 business days from sample receipt at Bloodworks NW HLA Lab.
    Available STAT?
    Yes, only by arrangement with Bloodworks Northwest HLA Lab. Call (206) 689-6580 (or 206-817-7730 after hours) for approval.
    Performing Location(s)
    Sendout Bloodworks Northwest Immunogenetics/HLA Laboratory
    206-689-6580

    921 Terry Ave
    6th Floor
    Seattle, WA 98104

    Laboratory staffed for questions: Monday - Friday, 07:00 a.m. to 05:00 p.m.
    After-Hours, Holidays, Weekends: Contact the Lead Technical Coordinator at (206) 817-7730
    Fax: 206-689-6582

    Billing & Coding

    CPT Codes
    86832, 86833
    LOINC
    34960-5