HLA Antibody Specificity (Sendout)
General Information
- Lab Name
- HLA Antibody Specificity (Sendout)
- Lab Code
- RHLAS
- Epic Name
- HLA Antibody Specificity (Sendout)
- External Test Id
- 3083-16C
- Description
Indications: Solid Organ Transplant.
Test Information: To identify and quantify HLA Antibody specificities. It can be used for potential donor selection and/or antibody reduction therapies.
- References
Bloodworks Northwest Test Information:
- Synonyms
- 3083-16, Calculated Percent Reactive Antibody, CPRA, HLA Antibody Identification
- Components
-
Code Name RHCPRA Calculated Percent Reactive Antibody (CPRA) RHUANT Unacceptable Antigens RHWKAB Weak Antibodies
Interpretation
- Method
Flow Cytometry using microparticles (“beads”) coated with recombinant or soluble HLA antigens
- Reference Range
- See individual components
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
10 mL blood in RED TOP (clot) tube
Also Accepted: 6 mL RED TOP (clot) tube
- Forms & Requisitions
- Bloodworks Nortwest Immunogenetics/HLA Laboratory Requisition (for orders not placed in Epic)
- Handling Instructions
Outside Laboratories:
- Store and transport whole blood at ambient temperature (serum from a red top tube is also accepted). Samples must be received by Bloodworks Northwest within 10 days of collection.
- A completed Bloodworks Northwest Immunogenetics/HLA Requisition must accompany all orders. In the Billing section, indicate "Bill to UWMC DLMP."
Stability: Ambient: 10 days.
- Quantity
-
Requested: 10 mL whole blood in a red top tube
Minimum: 2 mL whole blood in a red top tube
Processing
- Receiving Instructions
Store and transport samples at ambient temperature.
Sendouts:
- Bloodworks NW HLA Test: 3083-16
- Interfaced: Yes [Interface: 603; Worksheet: UBWHLA/HBWHLA/FBWHLA or other location-specific worksheet group]
- Send sample(s) with a copy of the Bloodworks Northwest HLA Requisition or the EPIC requisition (must indicate the test or panel to be ordered at Bloodworks NW).
- Package sample(s) with the associated requisition in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biohazard bag will be rejected by Bloodworks NW.
- Send routine samples via scheduled DLMP Courier shipments or Delivery Express, as needed.
- STAT orders should be transported to Bloodworks Northwest as received.* Depending on the courier schedule, it may be necessary to individually batch a STAT order and/or call Delivery Express for pickup.
- *STAT orders for this test are rare and require the provider to request approval from the HLA Lab. If approval is obtained, the HLA Lead Technical Coordinator will contact CSS to request expedited transport of a sample. CSS will create an HC1 task for SPS. If this is not done, orders should be considered routine.
Stability: Ambient: 10 days. Serum is also accepted.
- Misc Sendout
Performance
- Lab Department
- Sendouts BWNW Lab (AB)(BWAB)
- Frequency
- Performed: Performed: Monday through Friday. Reported: 5-7 business days from sample receipt at Bloodworks NW HLA Lab.
- Available STAT?
- Yes, only by arrangement with Bloodworks Northwest HLA Lab. Call (206) 689-6580 (or 206-817-7730 after hours) for approval.
- Performing Location(s)
-
Sendout Bloodworks Northwest Immunogenetics/HLA Laboratory
206-689-6580921 Terry Ave
6th Floor
Seattle, WA 98104Laboratory staffed for questions: Monday - Friday, 07:00 a.m. to 05:00 p.m.
After-Hours, Holidays, Weekends: Contact the Lead Technical Coordinator at (206) 817-7730
Fax: 206-689-6582
Billing & Coding
- CPT Codes
- 86832, 86833
- LOINC
- 34960-5