Genetic Renal Panel (Sendout)

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General Information

Lab Name
Genetic Renal Panel (Sendout)
Lab Code
RGENRP
Epic Name
Genetic Renal Panel - Iowa MORL (Sendout)
External Test Id
GRP08
Description

Test Information: The Genetic Renal Panel v8 platform includes 13 genes implicated in thrombotic microangiopathies (TMA), which includes complement-mediated Hemolytic Uremic Syndrome (aHUS), Upshaw-Schulman syndrome (a hereditary form of Thrombotic Thrombocytopenic Purpura (TTP)), methylmalonic aciduria (cobalamin deficiency) cblC type with homocystinuria, glucose-6-phosphate dehydrogenase (G6PD) deficiency, Denys-Drash syndrome (DDS). Many of the genes listed above have also been implicated in C3 Glomerulopathy (C3G) which includes Dense Deposit Disease (DDD) and C3 Glomerulonephritis (C3GN). Additionally, Genetic Renal Panel v8 screens for two single nucleotide variants that could compromise responsiveness to Eculizumab (NM_001735.3(C5):c.2653C>T, p.Arg885Cys and NM_001735.3(C5):c.2654G>A, p.Arg885His). The Genetic Renal Panel v8 also includes copy number variation screening of the CFH-CFHR5 genomic region using multiplex ligation-dependent probe amplification (MLPA).

  • Genes Included: CFH, CFI, CD46 (also known as MCP), CFB, CFHR5, C3, THBD, DGKE, PLG, ADAMTS13, MMACHC, G6PD and WT1

Ordering Requirements:

  • Orders originating from UW Medicine outpatient Renal Clinics (includes UW-MT, HMC) are pre-approved. All other orders require approval by the Laboratory Medicine Resident (LMR) on-call.
  • For orders placed in Epic, a separate MORL requisition is not required.
Forms & Requisitions
Synonyms
ADAMTS13, C3, C3 Glomerulonephritis (C3GN), C3 Glomerulopathy (C3G), CFB, CFH, CFHR5, CFI, Complement-mediated Hemolytic Uremic Syndrome (aHUS), Dense Deposit Disease (DDD), Denys-Drash syndrome (DDS), DGKE, G6PD, glucose-6-phosphate dehydrogenase (G6PD) deficiency, GRP08, MCP (CD46), Methylmalonic aciduria (cobalamin deficiency) cblC type with homocystinuria, MMACHC, MORL, PLG, THBD, Thrombotic microangiopathies (TMA), Thrombotic Thrombocytopenic Purpura (TTP), Upshaw-Schulman syndrome, WT1
Components
Code Name
RGENRR Genetic Renal Panel Result
RGENPL Genetic Renal Panel Performing Lab

Interpretation

Method

Massively Parallel Sequencing, MLPA

Reference Range
See individual components
Ref. Range Notes

See report.

Interferences and Limitations

Please note: Blood samples drawn from a bone marrow transplant patient will result in genetic results for the donor rather than the patient.

Ordering & Collection

Specimen Type
Blood
Collection

Collect one of the following specimen types:

Blood: 5 mL in LAVENDER TOP (EDTA) tube

Saliva: DNA Genotek, ORAGene Discover, OGR-500

  • Note: Oral collection kits are not available in the laboratory. Providers may order kits directly by contacting MORL at: morl@uiowa.edu.

Buccal Swabs:

  • 4 swabs minimum
  • DNA Genotek, OraCollect, OCD-100
  • Note: Oral collection kits are not available in the laboratory. Providers may order kits directly by contacting MORL at: morl@uiowa.edu.

Extracted DNA: 5µg DNA suspended in ≥ 50µl elution buffer

  • Minimum concentration 50ng/µl
  • A260/A280 1.8-2.0
Forms & Requisitions
Approval Required
Pre-approved for UW Medicine Outpatient Renal Clinics. Orders from other locations must be approved by the Lab Medicine Resident on-call.
Quantity
Requested: 5 mL EDTA whole blood
Minimum: 3 mL EDTA whole blood

Processing

Receiving Instructions

Store and transport whole blood at room temperature. Samples may be refrigerated if shipment is delayed.

Sendouts:

  • Order UIMORL Test: GRP08
  • For orders placed in Epic, a separate UIMORL requisition is not required. Print the full Epic requisition, ensuring that all pages are included. Current lab results (requested information from MORL) will print on the second page. CAST billing stickers are not required.
  • For orders not placed in Epic, the provider or care team must complete a MORL requisition for inclusion with the sample. Affix the CAST Billing sticker to the requisition to prevent accessioning delays.
  • Ship via FedEx Priority Overnight, Monday through Thursday only with a copy of the Epic or MORL requisition.

Stability:

  • Whole Blood: Ambient: 7 days; Refrigerated: 4 months; Frozen: Unacceptable.
  • Saliva Collection Kits and Buccal Swabs: OGR-500 saliva collection kits are stable at room temperature for several years and OCD-100 buccal swabs stable up to 80 days.
Misc Sendout

Performance

Lab Department
Sendouts(SO)
Frequency
TAT: 3 weeks.
Available STAT?
No
Performing Location(s)
Sendout University of Iowa, Molecular Otolaryngology and Renal Research Laboratories
319-335-6623

285 Newton Rd
5270 CBRB
Iowa City, IA 52242

Billing & Coding

CPT Codes
81479
LOINC
19145-2