Valproic Acid, Free and Total (Sendout)

General Information

Lab Name
Valproic Acid, Free and Total
Lab Code
RFTVPA
Epic Name
Valproic Acid, Free and Total (Sendout)
External Test Id
VALPG
Description

Useful For:

  • Monitoring both total and free valproic acid levels in therapy
  • Assessing compliance
  • Evaluating potential toxicity

Ordering Guidance: Order this test to obtain free and total valproic acid levels. For total valproic acid only, refer to Valproic Acid (Depakene) [VPA].

References
Synonyms
Depakene, Depakote, Divalproex, Free Valproic Acid, VALPG, Valproate
Components

Interpretation

Method

Valproic Acid, Total: Immunoassay Valproic Acid; Valproic Acid, Free: Ultrafiltration Followed by Immunoassay

Reference Range
See individual components
Ref. Range Notes

Reference Values:

Valproic Acid, Total:

Therapeutic: 50 (trough)-125 (peak) mcg/mL
Critical value: > or =151 mcg/mL

Valproic Acid, Free:

Therapeutic: 5-25 mcg/mL
Critical value: >30 mcg/mL

Interpretation:

The generally acceptable range for total valproic acid used as a reference to guide therapy is 50 to 125 mcg/mL. The corresponding range of free valproic acid concentration for clinical reference is 5 to 25 mcg/mL.

Low free valproic acid concentration relative to these ranges may suggest inadequate dosing, whereas a high free valproic acid concentration may be associated with toxic effects.

Because the concentration of valproic acid fluctuates considerably depending on the time from last dose, interpretation of the clinical significance of the valproic acid concentration must take into consideration the timing of the blood specimen. For this reason, 2 collections are sometimes made to assess the trough and peak concentrations.

Interferences and Limitations

Cautions: Specimens subjected to significant heat or other factors that could cause protein denaturation would demonstrate an artificially increased free valproic acid.

References

Ordering & Collection

Specimen Type
Blood
Collection

6 mL blood in GOLD TOP SST or RED TOP tube

Handling Instructions

Outside Laboratories: Centrifuge sample and aliquot serum into a separate plastic vial within 2 hours of collection. Refrigerate serum while awaiting shipment. Transport with a cold pack.

Stability: Refrigerated (preferred): 14 days; Frozen: 28 days; Ambient: 72 hours.

Reject Due To: Gross hemolysis.

Quantity
Requested: 2 mL serum
Minimum: 1 mL serum

Processing

Receiving Instructions

Centrifuge sample and aliquot serum into a separate plastic vial within 2 hours of collection. Refrigerate serum.

Sendouts:

  • Order Mayo Test: VALPG
  • Interfaced: Yes [Interface: 601; Worksheet: MARF]

Stability: Refrigerated (preferred): 14 days; Frozen: 28 days; Ambient: 72 hours.

Reject Due To: Gross hemolysis.

Misc Sendout

Performance

Lab Department
Sendouts Mayo Lab (RF)(MARF)
Frequency
Performed: Monday through Sunday. Reported: 1 day from sample receipt at Mayo Clinic Labs.
Available STAT?
No
Performing Location(s)
Sendout Mayo Clinic Laboratories
800-533-1710

200 First Street Southwest
Rochester, MN 55901

Billing & Coding

CPT Codes
80164, 80165
LOINC
4086-5
Interfaced Order Code
UOW5024