Brucella Ab Screen, IgG and IgM (Sendout)
General Information
- Lab Name
- Brucella Ab Screen,IgG and IgM (Sendout)
- Lab Code
- RFEBG
- Epic Ordering
- Brucella Ab, IgG and IgM (Sendout)
- External Test Id
- BRCMG
- Description
Useful for: Evaluating patients with suspected brucellosis.
Test Algorithm: If Brucella antibody screen, IgG and IgM is positive or equivocal, then confirmation will be performed at an additional charge.
- References
- Mayo Clinic Laboratories: Brucella Antibody Screen Clinical & Interpretive Information
- Synonyms
- BRCMG, Brucella abortus, Brucella Meletensis, Brucella Suis, Febrile Agglutinins
- Components
-
Code Name RBRCM Brucella Ab Screen, IgM RBRCG Brucella Ab Screen, IgG RFEBIN Brucella Ab Screen Interpretation
Interpretation
- Method
Enzyme Linked Immunosorbent Assay (ELISA)
- Reference Range
- See individual components
- Interferences and Limitations
Cautions:
This test utilizes antigen derived from Brucella abortus strain W99. However, significant cross-reactivity exists for other Brucella species (except B canis) and, therefore, the assays should not be used to differentiate infection at the species level.
B canis, a rare cause of brucellosis, may not be detected by this method.
Detection of specific IgM or IgG-class antibody to B melitensis and B suis by this method has not been determined.
ELISA tests are intended to be used as a screen only. Positive results should be followed up using an agglutination assay for confirmation. Results must be used in conjunction with symptoms, patient history, and other clinical findings.
B abortus strain RB51 is used for vaccination of animals in the US. There are currently no serologic tests to detect an antibody response to strain RB51 in humans. Per CDC guidelines, routine clinical serology tests for Brucella do not detect an antibody response to strain RB51. Note that other strains besides RB51 may be used for vaccinating animals outside of the US.
- References
- Mayo Clinic Laboratories: Brucella Antibody Screen Clinical & Interpretive Information
- Guidelines
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
3 mL blood in RED TOP or GOLD SST
- Handling Instructions
Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.
Stability: Refrigerated (preferred): 14 days; Frozen: 14 days; Ambient: Unacceptable.
Reject Due to: Gross hemolysis, gross lipemia, gross icterus,
heat-inactivated specimen.- Quantity
-
requested: 1.0 mL serum
minimum: 0.4 mL serum
Processing
- Processing
Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.
Sendouts:
- Order Mayo Test: BRCMG.
- Interfaced: Yes.
Stability: Refrigerated (preferred): 14 days; Frozen: 14 days; Ambient: Unacceptable.
Reject Due to: Gross hemolysis, gross lipemia, gross icterus,
heat-inactivated specimen.
Performance
- LIS Dept Code
- Sendouts Mayo Lab (RF) (MARF)
- Performing Location(s)
-
Sendout Mayo Clinic Laboratories (Superior Drive)
800-533-17103050 Superior Drive NW
Rochester, MN 55901 - Frequency
- Monday, Wednesday, Friday; 9 a.m. Analytic Time: 1 day (Additional days for agglutination result if reflex testing is required.)
- Available STAT?
- No
Billing & Coding
- CPT codes
- 86622x2
- Billing Comments
Reflex agglutination testing may be performed at additional charge.
- LOINC
- 24387-3
- Interfaced Order Code
- UOW2157