Brucella Ab Screen, IgG and IgM (Sendout)

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General Information

Lab Name
Brucella Ab Screen,IgG and IgM (Sendout)
Lab Code
RFEBG
Epic Ordering
Brucella Ab, IgG and IgM (Sendout)
External Test Id
BRCMG
Description

Useful for: Evaluating patients with suspected brucellosis.

Test Algorithm: If Brucella antibody screen, IgG and IgM is positive or equivocal, then confirmation will be performed at an additional charge.

References
  1. Mayo Clinic Laboratories: Brucella Antibody Screen Clinical & Interpretive Information
Synonyms
BRCMG, Brucella abortus, Brucella Meletensis, Brucella Suis, Febrile Agglutinins
Components

Interpretation

Method

Enzyme Linked Immunosorbent Assay (ELISA)

Reference Range
See individual components
Interferences and Limitations

Cautions:

This test utilizes antigen derived from Brucella abortus strain W99. However, significant cross-reactivity exists for other Brucella species (except B canis) and, therefore, the assays should not be used to differentiate infection at the species level.

B canis, a rare cause of brucellosis, may not be detected by this method.

Detection of specific IgM or IgG-class antibody to B melitensis and B suis by this method has not been determined.

ELISA tests are intended to be used as a screen only. Positive results should be followed up using an agglutination assay for confirmation. Results must be used in conjunction with symptoms, patient history, and other clinical findings.

B abortus strain RB51 is used for vaccination of animals in the US. There are currently no serologic tests to detect an antibody response to strain RB51 in humans. Per CDC guidelines, routine clinical serology tests for Brucella do not detect an antibody response to strain RB51. Note that other strains besides RB51 may be used for vaccinating animals outside of the US.

References
  1. Mayo Clinic Laboratories: Brucella Antibody Screen Clinical & Interpretive Information
Guidelines

Ordering & Collection

Specimen Type
Blood
Collection

3 mL blood in RED TOP or GOLD SST

Handling Instructions

Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Stability: Refrigerated (preferred): 14 days; Frozen: 14 days; Ambient: Unacceptable.

Reject Due to: Gross hemolysis, gross lipemia, gross icterus,
heat-inactivated specimen.

Quantity
requested: 1.0 mL serum
minimum: 0.4 mL serum

Processing

Processing

Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.

Sendouts:

  • Order Mayo Test: BRCMG.
  • Interfaced: Yes.

Stability: Refrigerated (preferred): 14 days; Frozen: 14 days; Ambient: Unacceptable.

Reject Due to: Gross hemolysis, gross lipemia, gross icterus,
heat-inactivated specimen.

Performance

LIS Dept Code
Sendouts Mayo Lab (RF) (MARF)
Performing Location(s)
Sendout Mayo Clinic Laboratories (Superior Drive)
800-533-1710

3050 Superior Drive NW
Rochester, MN 55901

Frequency
Monday, Wednesday, Friday; 9 a.m. Analytic Time: 1 day (Additional days for agglutination result if reflex testing is required.)
Available STAT?
No

Billing & Coding

CPT codes
86622x2
Billing Comments

Reflex agglutination testing may be performed at additional charge.

LOINC
24387-3
Interfaced Order Code
UOW2157