Direct Antiglobulin (Sendout)

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General Information

Lab Name
Dir. Antiglob Coombs Test (Sendout)
Lab Code
RDAT
Epic Name
Dir. Antiglob Coombs Test (Sendout)
External Test Id
3125-00
Description

Indications: Detects the presence of red blood cell-bound IgG and/or complement (C3) by agglutination using an antiglobulin (Coombs) reagent. Positive results can occur in patients with autoimmune hemolytic anemia, drug-induced immune hemolysis (e.g., alpha-methyldopa, procainamide), hemolytic transfusion reactions, or hemolytic disease of the newborn. Positive results can also occur due to non-specific IgG absorption (e.g., hypergamma-globulinemia), and occasionally in otherwise healthy individuals. Elution testing is usually indicated after a positive result with IgG is detected.

Ordering Note: Order this code for sendout testing to Bloodworks Northwest only. For testing performed by UW Medicine TSL, refer to Direct Antiglobulin Test [DAT].

Forms & Requisitions
Synonyms
3125-00, Coombs-Direct, DAT, Direct Antiglobulin Test
Components
Code Name
RDATR Direct Antiglob. Result (Sendout)
S2DAT Dir. Antiglob Coombs Testing Lab

Interpretation

Method

Standard test tube methodology, Hemagglutination

Reference Range
See individual components

Ordering & Collection

Specimen Type
Blood
Collection

Preferred: 6 mL blood in PINK TOP (EDTA) tube

  • Also Accepted: 6 mL blood in LAVENDER TOP (EDTA) tube
  • Pediatric Draw: 1 full 3 ml EDTA sample; neonate: 2 full 0.5 ml EDTA microtainers

Unacceptable: SST or GEL tubes

Dual Signatures Required:

  • At the time of collection, a two-person verification of positive patient identity must be performed in the presence of the patient by the person collecting the specimen and a second qualified staff member. This includes a verbal read back of the full legal name, spelling of the legal name, and MRN as it appears in the medical record.
  • Both people performing verification must sign or initial the sample label(s).
  • Write the collection date and time on the sample label.
  • Signatures of both verifiers are requested on the Bloodworks Northwest form. (This is not required for testing as long as dual verification is documented on the sample label.)
Forms & Requisitions
Quantity
Requested: 6 mL EDTA whole blood
Minimum: 6 mL EDTA whole blood

Processing

Receiving Instructions

Store and transport whole blood at ambient temperature.

  • Review the sample label(s) and (Epic or Bloodworks NW) requisition form for accuracy. Dual verification signatures are required on the sample label(s) only.
  • Package sample(s) with the associated requisition in a biohazard bag (one patient per bag). Orders received with multiple patient samples/requisitions in the same biozard bag will be rejected by Bloodworks NW.
  • Send samples as received to Bloodworks Northwest via DLMP or Delivery Express courier.
Misc Sendout

Performance

Lab Department
Transfusion Support(TS)
Frequency
Performed: Daily. Turn around Time: Weekdays: 24 hours from sample receipt at Bloodworks Northwest; Weekends: 48 hours.
Available STAT?
Yes
Performing Location(s)
Sendout Bloodworks Northwest Central TSL/Immunohematology Reference Laboratory

921 Terry Ave
A-Level
Seattle, WA 98104

Central Transfusion Support Lab (TSL): 206-689-6525

Immunohematology Reference Lab (IRL): 206-689-6534

Billing & Coding

CPT Codes
86880
LOINC
51006-5
Interfaced Order Code
UOW1800