Cytomegalovirus (CMV) Antibodies, IgM and IgG (Sendout)

Interpretation

Method

Electrochemiluminescence Immunoassay (ECLIA)

Reference Range

See individual components

Ref. Range Notes

Reference Values:

CMV IgM:	Negative
CMV IgG:	Negative

Reference values apply to all ages.

Interpretation:

Negative:	Negative for cytomegalovirus (CMV) IgM and IgG. False negative results may occur in immunocompromised patients.
Borderline:	Recommend follow-up testing in 10 to14 days if clinically indicated.
Positive IgG:	CMV IgG antibodies detected, which indicate recent or remote infection. These individuals may transmit CMV to susceptible individuals through blood and tissue products.
Positive IgM:	CMV IgM antibodies detected, which may indicate active or recent infection. Low level IgM antibodies may persist for more than 12 months following disease resolution.

Interferences and Limitations

Cautions:

Sera collected very early during the acute stage of infection may have undetectable levels of cytomegalovirus (CMV) IgM or IgG.

Performance characteristics have not been evaluated in immunocompromised or immunosuppressed individuals. Immunocompromised patients may have impaired immune responses and nonreactive IgM and IgG results may be due to delayed seroconversion and, therefore, do not rule out current infection.

The CMV IgM and IgG results should not be used alone to diagnose CMV infection. Results should be considered in conjunction with clinical presentation, patient history and other laboratory findings.

The performance characteristics of these assays have not been evaluated or established for blood or plasma donors, cord blood, patients undergoing immunosuppressive therapy, patients with other disorders leading to immune suppression, or for testing of neonates.

Immune complexes or other immunoglobulin aggregates present in patient specimens may cause increased nonspecific binding and produce false-positive results.

Potential cross-reactivity for CMV IgM may occur with specimens positive for Epstein-Barr virus viral capsid antigen IgM. Potential cross-reactivity with autoimmune markers and antibodies against influenza vaccination could not be ruled out.

Potential cross-reactivity for CMV IgG with varicella-zoster virus IgG, measles IgG, mumps IgG and parvovirus B19 IgG and could not be ruled out. The potential cross-reactivity with E. coli and autoimmune markers could not be ruled out.

Samples should not be taken from patients receiving therapy with high biotin doses (ie, > 5 mg/day) until at least 8 hours following the last biotin administration.

References

• Mayo Clinical & Interpretive Information: Cytomegalovirus Antibody, IgM and IgG, Serum

Ordering & Collection

Specimen Type

Blood

Collection

3 mL blood in RED TOP tube or GOLD SST

Handling Instructions

Outside Laboratories: Centrifuge sample and aliquot serum to a separate vial. Refrigerate serum while awaiting shipment. Transport with a cold pack.

Stability: Refrigerated (preferred): 28 days; Frozen: 90 days; Ambient: 7 days.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus, heat-inactivation, or additives (eg, biocides, antioxidants).

Quantity

Requested: 1 mL serum
Minimum: 0.7 mL serum

Processing

Receiving Instructions

Centrifuge sample and aliquot serum to a separate vial. Refrigerate serum.

Sendouts:

• Order Mayo Test: CMVAB
• Interfaced: Yes [Interface: 601; Worksheet: MARF]

Stability: Refrigerated (preferred): 28 days; Frozen: 90 days; Ambient: 7 days.

Reject Due To: Gross hemolysis, gross lipemia, gross icterus, heat-inactivation, or additives (eg, biocides, antioxidants).

Misc Sendout

Performance

Lab Department

Sendouts Mayo Lab (RF)(MARF)

Frequency

Performed: Monday through Saturday. Report Available: 1-3 days from sample receipt at Mayo Clinic Lab.

Available STAT?

No

Performing Location(s)

Sendout	Mayo Clinic Laboratories (Superior Drive) 800-533-1710 3050 Superior Drive NW Rochester, MN 55901

Billing & Coding

CPT Codes

86644, 86645

LOINC

24315-4

Interfaced Order Code

UOW3000