C3 Glomerulopathy Complement Panel (Sendout)

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General Information

Lab Name
C3 Glomerulopathy Complement Panel (Sendout)
Lab Code
RC3GCP
Epic Name
C3 Glomerulopathy Complement Panel (Sendout)
External Test Id
C3G-CP
Description

Test Information: The C3G Functional Panel includes 21 pathway, autoantibody, and biomarker tests implicated in C3 Glomerulopathy (C3G), which includes Dense Deposit Disease (DDD) and C3 Glomerulonephritis (C3GN).

  • Tests Included: Fluid Phase Activity Assay (IFE), FHAA, FBAA, C3Nef (C3CSA), C5Nef (C3CSAP), C4Nef, C3, C3c, C4, FB, Ba, Bb, C5, FD, Properdin levels, Soluble C5b-9 (sC5b-9), FI level, FH level, CH50, AFPA, C3b Deposition Assay.

Ordering Note: Do not order concurrently with aHUS (TMA) Functional Panel (Sendout) [RAHUSP]. The C3 Glomerulopathy Complement Panel is more comprehensive and includes all tests in the aHUS TMA Panel.

Ordering Requirements:

  • Pre-approved for outpatient UW Medicine Renal Clinics (includes UW-MT, HMC). All other orders require Laboratory Medicine Resident (LMR) approval.
  • For orders placed in Epic, a separate MORL requisition is not required.
Forms & Requisitions
Synonyms
07C3GFP, AFPA (Alternative Pathway Functional Assay), Ba (Complement Ba Fragment Level), Bb (Complement Bb Fragment Level), C3 (Complement C3 Level), C3 Glomerulonephritis (C3GN), C3b Deposition Assay, C3c (Complement C3c Fragment), C3G Functional Panel, C3G-CP, C3Nef (C3CSA) (C3 Nephritic Factor), C4 (Complement C4 Level), C4Nef (C4 Nephritic Factor), C5 (Complement C5 Level), C5Nef (C3CSAP) (C5 Nephritic Factor), CH50, Dense Deposit Disease (DDD), FB (Complement Factor B Level), FBAA (Factor B Autoantibody), FD (Factor D Level), FH Level (Complement Factor H Level), FHAA (Factor H Autoantibody), FI Level (Complement Factor I Level), Fluid Phase Activity Assay, Properdin Level, Soluble C5b-9 (sC5b-9)
Components
Code Name
RC3GCR C3 Glomerulopathy Complement Panel Result
RC3GCL C3 Glomerulopathy Complement Panel Lab

Interpretation

Method

CH50: Liposome-based immunoassay

AFPA, FH Autoantibody, FB Autoantibody, FH Level, FI Level, C3c Level, Ba Level, Bb Fragment Level, Factor D Level, Properdin Level, sC5b-9, C5 Level: Enzyme Linked Immuno-Sorbent Assay (ELISA)

C3b Deposition Assay: Cell-based assay

Fluid Phase Activity Assay (IFE): Immunofixation

C3NeF (C3CSA), C5NeF (C3CSAP), C4NeF: Hemolytic assay

C3 Level, C4 Level: Turbidimetry-based method

FB Level: Radial immunodiffusion (RID)

Reference Range
See individual components
Ref. Range Notes

See report.

Ordering & Collection

Specimen Type
Blood
Collection

6 mL blood in RED TOP tube AND 6 mL blood in LAVENDER TOP (EDTA) tube

Unacceptable: GOLD SST

Forms & Requisitions
Approval Required
Pre-approved for UW Medicine Outpatient Renal Clinics. Orders from other locations must be approved by the Lab Medicine Resident on-call.
Quantity
Requested: 2 mL serum and 2 mL plasma
Minimum: 2 mL serum and 2 mL plasma

Processing

Receiving Instructions

Centrifuge samples. Transfer plasma and serum to separate plastic vials and freeze at -70°C.

Sendouts:

  • Order UIMORL Test: C3G-CP.
    Label samples clearly as "serum" or "plasma."
  • For orders placed in Epic, a separate UIMORL requisition is not required. Print the full Epic requisition, ensuring that all pages are included. Current lab results (requested information from MORL) will print on the second page. CAST billing stickers are not required.
  • For orders not placed in Epic, the provider or care team must complete a MORL requisition for inclusion with the sample. Affix the CAST Billing sticker to the requisition to prevent accessioning delays.
  • Ship FedEx Priority Overnight, Monday through Thursday only, with sufficient dry ice to ensure that samples remain frozen throughout transport.
    • Package samples as close to FedEx pickup as possible to ensure that samples remain frozen upon arrival at UIMORL.
    • Samples that arrive thawed will be rejected.

Stability: Frozen (-70°C): Required; Refrigerated: Unacceptable; Ambient: Unacceptable.

Misc Sendout

Performance

Lab Department
Sendouts(SO)
Frequency
TAT: 4 weeks
Available STAT?
No
Performing Location(s)
Sendout University of Iowa, Molecular Otolaryngology and Renal Research Laboratories
319-335-6623

285 Newton Rd
5270 CBRB
Iowa City, IA 52242

Billing & Coding

CPT Codes
83516x2, 86160x12, 86161x5, 86162, 86334
LOINC
18727-8