aHUS (TMA) Functional Panel (Sendout)

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General Information

Lab Name
aHUS (TMA) Functional Panel (Sendout)
Lab Code
RAHUSP
Epic Name
aHUS (TMA) Functional Panel (Sendout)
External Test Id
aHUS-FP
Description

Test Information: The aHUS TMA Funtional Panel includes 18 functional and biomarker tests implicated in thrombotic microangiopathies (TMA), which include complement-mediated hemolytic uremic syndrome (aHUS) and Upshaw-Schulman syndrome, a hereditary form of Thrombotic Thrombocytopenic Purpura (TTP).

  • Tests Included: C3, C3c, C4, C5, FD, FB, Ba, Bb, Properdin levels, Soluble C5b-9 (sC5b-9), FI and FH levels, CH50, APFA, FHAA, FBAA, C3b Deposition, Fluid Phase Activity-IFE.

Ordering Note: Do not order concurrently with C3 Glomerulopathy Complement Panel (Sendout) [RC3GCP]. All tests in the aHUS (complement-mediated TMA) panel are included in the more comprehensive C3 Glomerulopathy Complement Panel.

Ordering Requirements:

  • Pre-approved for outpatient UW Medicine Renal Clinics (includes UW-MT, HMC). All other orders require LMR approval.
  • For orders placed in Epic, a separate MORL requisition is not required.
Forms & Requisitions
Synonyms
aHUS (complement-mediated TMA) Panel, aHUS-FP, APFA (Alternative Pathway Functional Assay), Atypical Hemolytic Uremic Syndrome, Ba (Complement Ba Fragment Level), Bb (Complement Bb Fragment Level), C3 (Complement C3 Level), C3b Deposition, C3c (Complement C3c Fragment), C4 (Complement C4 Level), C5 (Complement C5 Level), CH50 Level, Complement-mediated Thrombotic Microangiopathy, FB (Complement Factor B Level), FBAA (Factor B Autoantibody), FD (Factor D Level), FH (Complement Factor H Level), FHAA (Factor H Autoantibody), FI (Complement Factor I Level), Fluid Phase Activity-IFE, Properdin Level, Soluble C5b-9 (sC5b-9), Thrombotic Thrombocytopenic Purpura (TTP), TMA Functional Panel
Components
Code Name
RAHUSR aHUS (TMA) Panel Result
RAHUSL aHUS (TMA) Panel Performing Lab

Interpretation

Method

C3, C4: Turbidimetry-based method

C3c, Ba, Bb, C5, FD, Properdin, Soluble C5b-9 (sC5b-9), FI and FH levels, APFA, FHAA, FBAA: Enzyme Linked Immuno-Sorbent Assay (ELISA)

FB: Radial immunodiffusion (RID)

CH50: Liposome-based immunoassay

C3b Deposition: Cell-based assay

Fluid Phase Activity-IFE: Immunofixation

Reference Range
See individual components
Ref. Range Notes

See report.

Ordering & Collection

Specimen Type
Blood
Collection

6 mL blood in RED TOP tube AND 6 mL blood in LAVENDER TOP (EDTA) tube

Unacceptable: GOLD SST

Forms & Requisitions
Approval Required
Pre-approved for UW Medicine Outpatient Renal Clinics. Orders from other locations must be approved by the Lab Medicine Resident on-call.
Quantity
Requested: 2 mL serum and 2 mL plasma
Minimum: 2 mL serum and 2 mL plasma

Processing

Receiving Instructions

Centrifuge samples. Transfer plasma and serum to separate plastic vials and freeze at -70°C.

Sendouts:

  • Order UIMORL Test: aHUS-FP.
  • Label samples clearly as "serum" or "plasma."
  • For orders placed in Epic, a separate UIMORL requisition is not required. Print the full Epic requisition, ensuring that all pages are included. Current lab results (requested information from MORL) will print on the second page. CAST billing stickers are not required.
  • For orders not placed in Epic, the provider or care team must complete a MORL requisition for inclusion with the sample. Affix the CAST Billing sticker to the requisition to prevent accessioning delays.
  • Ship FedEx Priority Overnight, Monday through Thursday only, with sufficient dry ice to ensure that samples remain frozen throughout transport.
    • Package samples as close to FedEx pickup as possible to ensure that samples remain frozen upon arrival at UIMORL.
    • Samples that arrive thawed will be rejected.

Stability: Frozen (-70°C): Required; Refrigerated: Unacceptable; Ambient: Unacceptable.

Misc Sendout

Performance

Lab Department
Sendouts(SO)
Frequency
TAT: 4 weeks
Available STAT?
No
Performing Location(s)
Sendout University of Iowa, Molecular Otolaryngology and Renal Research Laboratories
319-335-6623

285 Newton Rd
5270 CBRB
Iowa City, IA 52242

Billing & Coding

CPT Codes
83516x2, 86160x12, 86161x2, 86162, 86334
LOINC
18727-8