Bordetella pertussis/parapertussis PCR

This test replaces B. pertussis and B. parapertussis PCR (Sendout) [RBPRP]

General Information

Lab Name

Lab Code

BPPCR

Epic Name

Bordetella pertussis/parapertussis PCR

Description

Diagnostic test for the detection of Bordetella pertussis or Bordetella parapertussis

B. pertussis PCR is preferred to culture when a diagnostic test is required and detects roughly twice as many cases as culture. B pertussis DNA can be detected up to 4 weeks, or longer after symptom onset. See the CDC for more information on testing for B. pertussis.

Synonyms

B. parapertussis, B. pertussis, Bordetella parapertussis, Bordetella pertussis, BPRP, Pertussis PCR, Whooping Cough

Components

Code	Name
BPSPC	Bordetella pertussis/parapertussis PCR Specimen
BPRPR	Bordetella pertussis PCR Result
BPPRPR	Bordetella parapertussis PCR Result

Interpretation

Guidelines

Washington DOH Notifiable Conditions

Method

Real-time polymerase chain reaction (PCR) based qualitative in virto diagnostic test for the direct detection and identification of Bordetella pertussis and Bordetella parapertussis nucleic acid in a nasopharyngeal swab specimen

The DiaSorin Bordetella assay targets the Insertion sequences IS481 for B. pertussis and IS1001 for B. parapertussis.

Limitation: The IS481 is also found in B. holmesii.

Negative results do not preclude B. pertussis or B. parapertussis infection and positive results do not rule out co-infections with other respiratory pathogens.

Reference Range

See individual components

Ref. Range Notes

Reference Ranges:

Bordetella pertussis: Negative
Bordetella parapertussis: Negative

Interpretation: A positive result indicates the presence of DNA from Bordetella pertussis or B parapertussis.

A negative result indicates the absence of detectable B pertussis and B parapertussis DNA in the specimen but does not negate the presence of organism or active or recent disease and may occur due to inhibition of PCR (known inhibition rate of <1%), sequence variability or the presence of B pertussis or B parapertussis in quantities less than the limit of detection of the assay. Additionally, patients presenting late after symptom onset may test negative; in such cases, testing for B pertussis antibody, IgG, in serum may be considered.

Ordering & Collection

Specimen Type

Nasal Swab

Collection

Collection Note: The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Bordetella pertussis or Bordetella parapertussis DNA is unlikely.

Collect one of the following:

Nasopharyngeal swab (Flocked, Rayon or Polyester) placed in Copan Universal Transport Media (UTM) or an ESwab
Reject Due To: Nose, nasal, or throat swab; calcium alginate or cotton-tipped swab; swab sent in gel transport medium, or Regan Lowe media; swabs with solid plastic shaft; dry swab.

Handling Instructions

Refrigerate sample in original collection container.

Stability: Refrigerated (preferred): 7 days; Frozen: 7 days.

Reject Due To: Nose, nasal, or throat swab; calcium alginate or cotton-tipped swab; swab sent in gel transport medium, viral/universal transport medium, or Regan Lowe media; ; swabs with solid plastic shaft; dry swab.

Quantity

Requested: NP swab
Minimum: NP swab

Processing

Receiving Instructions

Misc Sendout

Performance

Lab Department

Serology(SR)

Frequency

Performed Monday to Sunday

Available STAT?

Performing Location(s)

UW-MT	Microbiology 206-520-4600 Clinical Lab, Room # NW177, University of Washington Medical Center, 1959 NE Pacific street, Seattle, WA 98195

Billing & Coding

CPT Codes: 87798x2
LOINC: 90441-7
Interfaced Order Code: UOW4718