AML1 ETO t(8:21) PCR (Sendout)

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General Information

Lab Name
AML1 ETO t(8:21) PCR
Lab Code
RAML1E
Epic Name
AML1 ETO t(8:21) PCR (Sendout)
Description

Test Description: Detection and quantitative monitoring of t(8;21) in AML.

    Ordering Requirements:

    • A completed HematoLogics Test Requisition Form should accompany all orders. The billing information will be entered by lab staff.
    References
    Forms & Requisitions
    Synonyms
    8:21, AML-ETO, RUNX1-RUNX1T1, RUNX1-RUNX1T1 (AML1-ETO) Translocation, t(8;21), t(8;21) by RT-PCR, t8:21
    Components
    Code Name
    RAML1S AML1 ETO t(8:21) Specimen
    RAML1R AML1 ETO t(8:21) Result
    RAML1I AML1 ETO t(8:21) Interp
    RAML1C AML1 ETO t(8:21) Comment

    Interpretation

    Method

    Real-Time Quantitative PCR

    Reference Range
    See individual components
    Ref. Range Notes

    See report.

    References

    Ordering & Collection

    Specimen Type
    Whole Blood, Bone Marrow
    Collection

    Blood: 2 mL blood LAVENDER TOP (EDTA) tube

    • Also accepted: 2 mL blood in Green top (Sodium Heparin) tube

      Bone Marrow: 2 mL bone marrow aspirate in LAVENDER TOP (EDTA) tube

      • Also accepted: 2 mL bone marrow in Green top (Sodium Heparin) tube
      Forms & Requisitions
      Quantity
      Requested: 2 mL whole blood or bone marrow
      Minimum: 2 mL whole blood or bone marrow

      Processing

      Receiving Instructions

      Store and transport peripheral blood or bone marrow samples at room temperature.

        Result at Entry:

        • RAML1S (AML1 ETO t(8:21) Specimen): <Enter the appropriate specimen type code from the table below.>

        Specimen Type Codes:

        ETC Translation
        WB Whole Blood
        BMASP Bone Marrow Aspirate

        UW-MT Sendouts:

        • Hematologics Test: RUNX1::RUNX1T1 t(8;21) [listed under "Molecular Studies"]
        • If a Hematologics Test Requisition Form does not accompany the sample, it may be completed by lab staff.
          • Include a copy of the Epic requisition. The physician authorization/time stamp on the Epic order can be submitted in lieu of a physical physician signature if accompanied by a Hematologics requisition.
        • Send samples Monday through Friday via Delivery Express. Samples must be received at Hematologics within 5 days of collection. Same-day or next-day transport is preferred.

        Hematopathology SMG:

        • If ordered in conjunction with other Hematopathology testing, samples may be sent out directly to Hematologics, Inc. to avoid additional transport delays. Refer to sendout instructions above.

        Stability: Ambient (Preferred): 5 days; Refrigerated: Not recommended; Frozen: Unacceptable.

        • Note: Sample viability may be compromised by refrigeration.
        Misc Sendout

        Performance

        Lab Department
        Sendouts(SO)
        Frequency
        Performed: Monday through Friday. May be performed on Saturday by special arrangement; contact Hematologics at 206-223-2700. Turn around Time: 24-72 hours from sample receipt at Hematologics.
        Available STAT?
        No
        Performing Location(s)
        Sendout Hematologics, Inc.
        206-223-2700; 206-264-4459; 800-860-0934

        3161 Elliott Ave.
        Suite 200
        Seattle, WA 98121

        Fax: 206-223-5550
        Email: clientservices@hematologics.com
        After Hours Paging: 206-264-4459
        Hours: Monday through Friday: 7:00 am - 5:00 pm; Saturday: 7:00 am to 2:00pm. Closed Sundays.

        Billing & Coding

        CPT Codes
        81401
        LOINC
        72207-4