DNase B Antibody Titer (Sendout)
General Information
- Lab Name
- DNase B Antibody Titer
- Lab Code
- RADNAS
- Epic Ordering
- DNAse B Antibody Titer (Sendout)
- External Test Id
- ADNAS
- Description
Useful For: Demonstration of acute or recent streptococcal infection using anti-DNase B titer.
- References
- Mayo Clinic Laboratories Anti-DNase B Titer, Serum Clinical & Interpretive Information
- Synonyms
- ADB, ADNAS, Anti-DNase B, Antideoxyribonuclease B, DBA Streptococcal Antibody, Streptococcal Antibodies
Interpretation
- Method
Nephelometry
- Reference Range
-
Units: U/mL
Female Male Age Range Age Range 0-4y 0-250 0-4y 0-250 5y-17y 0-375 5y-17y 0-375 18y- 0-300 18y- 0-300 Effective date: 11/01/2017
- Ref. Range Notes
Interpretation: Elevated values are consistent with an antecedent infection by group A streptococci. Although the antistreptolysin O (ASO) test is quite reliable, performing the anti-DNase is justified for 2 primary reasons. First, the ASO response is not universal. Elevated ASO titers are found in the sera of about 85% of individuals with rheumatic fever; ASO titers remain normal in about 15% of individuals with the disease. The same holds true for other streptococcal antibody tests: a significant portion of individuals with normal antibody titers for 1 test will have elevated antibody titers for another test. Thus, the percentage of false-negative results can be reduced by performing 2 or more antibody tests. Second, skin infections, in contrast to throat infections, are associated with a poor ASO response. Patients with acute glomerulonephritis following skin infection (post-impetigo) have an attenuated immune response to streptolysin O. For such patients, performance of an alternative streptococcal antibody test, such as this assay, is recommended.
- Interferences and Limitations
Cautions: False-high titers may be obtained with sera that are contaminated by certain bacterial organisms during shipment or storage and in patients with liver disease where the presence of high lipoprotein concentrations in the serum may mimic antibody activity.
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
Patient Preparation: Fasting preferred, but not required.
Collection: 3 mL blood in GOLD SST or RED TOP tube
- Handling Instructions
Outside Laboratories: Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.
Stability: Refrigerated (preferred): 28 days; Frozen: 28 days; Ambient: 7 days.
Reject Due To: Gross lipemia. Gross hemolysis, gross icterus are acceptable.
- Quantity
-
requested: 1 mL serum
minimum: 0.5 mL serum
Processing
- Processing
Centrifuge sample and transfer serum to a separate plastic vial. Refrigerate serum.
Sendouts:
- Order Mayo Test: ADNAS.
- Interfaced: Yes.
Stability: Refrigerated (preferred): 28 days; Frozen: 28 days; Ambient: 7 days.
Reject Due To: Gross lipemia. Gross hemolysis, gross icterus are acceptable.
Performance
- LIS Dept Code
- Sendouts Mayo Lab (RF) (MARF)
- Performing Location(s)
-
Sendout Mayo Clinic Laboratories (Superior Drive)
800-533-17103050 Superior Drive NW
Rochester, MN 55901 - Frequency
- Performed: Monday - Friday. Report Available: 2-5 days.
- Available STAT?
- No
Billing & Coding
- CPT codes
- 86215
- LOINC
- 5133-4
- Interfaced Order Code
- UOW3477
- Interfaced Result Code
- UOW3477