URD Ab Titer Not Anti A or B (Sendout)

Indications: Determination of the titer of clinically significant IgG (non-ABO) antibodies present in the patient’s plasma. Antibody Titration is also performed to confirm the presence HTLA antibodies.

Test Information: This test is most often performed on the plasma of pregnant women which contains an antibody(ies) implicated in causing Hemolytic Disease of the fetus and newborn (HDFN). Serial, two-fold dilutions of serum are tested against red cells carrying the antigen of interest (e.g., D, K) using the indirect antiglobulin method. Result is expressed as the reciprocal of the highest serum dilution which still shows (1+) agglutination. This test is used to monitor serum alloantibody levels in previously sensitized pregnant women for prenatal management of hemolytic disease of the newborn (HDN).Testing may be repeated at intervals throughout the pregnancy to monitor for a significant increase in titer. In women with Rh alloimmunization, anti-D levels greater or equal to 16 are generally considered an indication for invasive fetal testing.

Ordering Note: This test is for antibody titer other than Anti A or Anti B. For Anti A titer, see test Anti A Titer (Sendout) [RAT]. For Anti B titer, see test Anti B Titer (Sendout) [RBT].

7 mL whole blood in EDTA LAVENDER TOP tube

Unacceptable: Serum separator gel tube

Maintain whole blood at ambient temperature.

Send samples to Bloodworks Northwest with the completed Bloodworks Requisition or a copy of the Epic requisition.