Amino Acids, Plasma (Sendout)

General Information

Lab Name
Amino Acids, PLASMA
Lab Code
RAA
Epic Name
Amino Acids, Plasma
External Test Id
LAB811
Description

Description: Measurement of 25 amino acids by ion exchange chromatography. This test includes the following: Taurine, Aspartic Acid, Hydroxyproline, Threonine, Serine, Asparagine, Glutamic Acid, Glutamine, Proline, Glycine, Alanine, Citrulline, Valine, Cystine, Methionine, Alloisoleucine, Isoleucine, Leucine, Tyrosine, Phenylalanine, Homocystine, Ornithine, Lysine, Histidine, Arginine.

Clinical Utility: Amino acid analysis can be useful in the clinical work up, diagnosis, or dietary monitoring of the following disorders: Phenylketonuria, tyrosinemia, maple syrup urine disease, urea cycle defects (argininosuccinic aciduria, argininemia, CPS, OTC deficiency, citrullinemia), non-ketotic hyperglycinemia, homocystinuria, defects of cobalamin metabolism, cystinuria (urine only), ornithinemia, and lysinuric protein intolerance. Amino acid analysis is also performed for nutritional monitoring.

Ordering Requirements: Include relevant clinical information (drug therapy, family history, etc.) in the Epic order comment to ensure proper interpretation.

References
Synonyms
AAQNT, Alanine, Alloisoleucine, Arginine, Asparagine, Aspartic Acid, Citrulline, citrullinemia, Cystine, Glutamic Acid, Glutamine, Glycine, Histidine, Homocystine, Hydroxyproline, hyperglycinemia, Isoleucine, LAB811, Leucine, Lysine, Methionine, NKH, Ornithine, Phenylalanine, Proline, Serine, Taurine, Threonine, Tyrosine, Valine
Components

Interpretation

Method

Ion exchange chromatography with post column derivatization

Reference Range
See individual components
Ref. Range Notes

Refer to Plasma Amino Acid Reference Ranges.

Interferences and Limitations

Note: Amino Acids performed on urine samples is not clinically indicated.

References

Ordering & Collection

Specimen Type
Blood
Collection

3 mL blood in DARK GREEN (Na Heparin) or LIME GREEN PST (Lithium Heparin)

Also Accepted: RED or LAVENDER TOP (EDTA) tube. Note: Serum or EDTA plasma is acceptable, but not preferred.

Collection Timing: For infants and children, draw sample prior to feeding or 2-3 hours after a meal.

Handling Instructions

Outside Laboratories: Centrifuge sample and transfer plasma/serum to a separate plastic vial within one hour of collection. Freeze plasma/serum at -20°C while awaiting shipment. Transport on dry ice.

  • Optional: To ensure proper interpretation, include relevant clinical information (drug therapy, and family history) on the DLMP requisition.

Stability: Frozen (preferred): 1 month; Refrigerated: 24 hours; Ambient: 2 hours.

Quantity
Requested: 1 mL plasma or serum
Minimum: 0.5 mL plasma or serum

Processing

Receiving Instructions

Centrifuge sample and transfer plasma/serum to a separate plastic vial within one hour of collection. Freeze plasma/serum at -20°C.

SPS, order this test on a separate accession.

Sendouts:

  • Order SEACHL Test: LAB811
  • Interfaced: Yes [Interface: 601; Department: SOSC]
  • If clinical information (drug therapy, family history) is included in the order, print the Epic requisition and send it with a copy of the MayoAccess Batch Sheet.
  • Send samples on dry ice via DLMP or DE courier.

Stability: Frozen: 1 month; Refrigerated: 24 hours; Ambient: 2 hours.

Misc Sendout

Performance

Lab Department
SCHL Interfaced Sendouts(SOSC)
Frequency
Performed: Monday through Friday. TAT: 2-5 days from sample receipt at Seattle Children's Lab.
Available STAT?
No
Performing Location(s)
Sendout Seattle Children's Hospital Laboratories
206-987-2617

4800 Sand Point Way NE
Seattle, WA 98105

Room Number: FB.2.441

Other Locations/Notes

Seattle Children's Hospital Laboratory, Biochemical Genetics: 206-987-2216

Billing & Coding

CPT Codes
82139
LOINC
12468-5
Interfaced Order Code
UOW5040