BCR/ABL p210, Quant Reflexive
General Information
- Lab Name
- BCR/ABL p210, Quant Reflexive
- Lab Code
- QP210R
- Epic Ordering
- BCR/ABL p210, Quant Reflexive
- Description
This assay is a nucleic acid amplification test for the quantitation of BCR::ABL1 p210 transcripts (e13a2 and e14a2) in total RNA from whole blood or bone marrow aspirate. This quantitative test is recommended for patients with uncertain diagnosis or unknown forms of BCR::ABL1 fusion. The test does not differentiate between e13a2/b2a2 or e14a2/b3a2 fusion transcripts and does not detect other rare fusion transcripts resulting from t(9;22). For samples with not detected BCR::ABL1 p210 transcripts, BCR::ABL1 p190, Quant by RT PCR [QP190] will be ordered.
To facilitate the interlaboratory comparison of findings and the assessment of molecular milestones (major molecular response or MMR), results are reported using the International Scale.
The reportable range is 0.0030% to 50% IS
The limit of detection is 0.0030% IS.
Stability is 72 hrs. Specimens received after 72 hrs. will be rejected.Limitations: This assay in unable to detect BCR::ABL1 p190, p230, or other rare transcripts.
Please complete the appropriate request form based on your patient location. Hematopathology test request form can be found at Clinical Pathology Test Order Forms.
- Synonyms
- B-ALL, B-Cell Acute Lymphoblastic Leukemia, BALL, BCR::ABL1, Chronic Myelogenous Leukemia, CML, P210, QP190, QP210, Quantitative BCR/ABL p210, t(9;22)
- Components
-
Code Name P210SD BCR/ABL p210 Specimen Desc P210RS BCR/ABL p210 Result P210IS BCR/ABL p210 %IS P210IN BCR/ABL p210 Interpretation P210M BCR/ABL p210 Method P210GR P210 Case Number
Interpretation
- Method
Real-Time PCR
- Reference Range
- See individual components
- Guidelines
Ordering & Collection
- Specimen Type
- Peripheral Blood or Bone Marrow Aspriate
- Collection
-
Required:
Peripheral blood or Bone Marrow Aspirate collected in EDTA
Unacceptable:
Serum, plasma, extracted DNA, CSF, tissue, frozen whole blood or bone marrow aspirate. Specimens collected in anticoagulants other than EDTA. Severely hemolyzed or clotted specimens.
Specimens received beyond 72hr stability limit.
- Forms & Requisitions
- Handling Instructions
Samples should be refrigerated and transported to the lab without delay and arrive in lab within 24 hours.
The stability limit is 72 hours, samples beyond this limit will be rejected.
- Quantity
-
requested: Blood: 6 mL in EDTA; Bone Marrow Aspirate: 1-2mL in Lavender Top (EDTA)
minimum: Blood: 4 mL in EDTA; Bone Marrow Aspirate: 1mL in Lavender Top (EDTA)
Processing
- Processing
Send specimens to Hematopathology – Molecular department for processing.
Performance
- LIS Dept Code
- Molecular Hematopathology (MHP)
- Performing Location(s)
-
Other Hematopathology, Molecular
206-606-7060UW Hematopathology Laboratory, G7-800
Fred Hutchinson Cancer Center
825 Eastlake Avenue E.
Seattle, WA 98109Hematopathology, Molecular hours:
7:00 am - 5:30 pm M-F
8:00 am - 4:30 pm Saturday, closed on Sunday, and with reduced staffing on major holidays - Frequency
- BCR/ABL1 p210 Monday – Friday, BCR/ABL1 p190 Monday, Wednesday, Friday. Results available within 96hrs from receipt..
- Available STAT?
- No
Billing & Coding
- CPT codes
- 81206
- LOINC
- 21822-2
- Interfaced Order Code
- UOW3789