BCR/ABL p190, Quant by RT PCR
General Information
- Lab Name
- BCR/ABL p190, Quant by RT PCR
- Lab Code
- QP190
- Epic Ordering
- BCR/ABL p190 Quantitative
- Description
The Philadelphia chromosome (Ph) is the product of the reciprocal translocation between the ABL1 gene on chromosome 9, and the BCR gene on chromosome 22. Depending on variations in splicing, the BCR exon 1 can join to the ABL1 exon 2, resulting in the p190 (e1:a2) BCR/ABL1 breakpoint. p190 is found almost exclusively in Ph+ALL patients, but can, on occasion, be found in CML patients as an alternative splice variant. Our p190 assay is a laboratory-developed test using real- time quantitative PCR.
Reportable Range: 0.01 – 100%
Limit of Detection: 0.002%
Limit of Quantification: 0.01%
Precision near LOD (CV): 24%Stability is 4 days, specimens received after 4 days will be rejected.
Hematopathology test request form can be found at Request Form.
- Forms & Requisitions
- Synonyms
- B-ALL, B-Cell Acute Lymphoblastic Leukemia, BALL, P190, QP190, Quantitative BCR/ABL p190, t(9;22)
- Components
-
Code Name P190SD BCR/ABL p190 Specimen Desc P190RS BCR/ABL p190, Quant by RT PCR P190RA BCR/ABL p190 Ratio % P190IN BCR/ABL p190 Interpretation P190M BCR/ABL p190 Method P190GR P190 Case Number
Interpretation
- Method
LDT, RT-PCR
- Reference Range
- See individual components
- Guidelines
Ordering & Collection
- Specimen Type
- Peripheral blood, Bone Marrow aspirate
- Collection
-
Blood: 6mL in LAVENDER top (EDTA) (minimum total of 5 million WBC)
Bone Marrow: 1-2 mL in a LAVENDER top (EDTA) (minimum total of 5 million WBC)Unacceptable:
Specimens with low volume or inadequate WBC.
Specimens received beyond stability limit.
Tissue, Decalcified or unacceptable fixativesPlease note: While green top heparin tubes are accepted for testing, there is documentation that heparin can interfere with some PCR.
- Forms & Requisitions
- Handling Instructions
Samples should be transported at RT and arrive in lab within 24 hours.
If sample is expected to arrive in the HP lab beyond 24 hours, store and transport at 2 – 6˚C.
- Quantity
-
requested: Blood: 6mL in EDTA, Bone Marrow: 2mL in EDTA
minimum: Blood: 4mL in EDTA, Bone Marrow: 1mL in EDTA
Processing
- Processing
Send specimens to Hematopathology – Molecular department for processing.
The stability limit is 4 days, samples beyond this limit will be rejected.
Performance
- LIS Dept Code
- Molecular Hematopathology (MHP)
- Performing Location(s)
-
Other Hematopathology, Molecular
206-606-7060UW Hematopathology Laboratory, G7-800
Fred Hutchinson Cancer Center
825 Eastlake Avenue E.
Seattle, WA 98109Hematopathology, Molecular hours:
7:00 am - 5:30 pm M-F
8:00 am - 4:30 pm Saturday, closed on Sunday, and with reduced staffing on major holidays - Frequency
- Monday, Wednesday, Friday: Results available within 96hrs from receipt.
- Available STAT?
- No
Billing & Coding
- CPT codes
- 81207
- LOINC
- 21823-0
- Interfaced Order Code
- UOW2718