Lead & Zinc Protoporph/Heme Ratio

General Information

Lab Name

Pb and ZPPH

Lab Code

PBGRP

Epic Name

Lead Level w/ZPPH

Description

Use: Increased ZPPH levels can be observed in iron deficiency and chronic lead poisoning.

Synonyms

Chronic lead poisoning, PB

Components

Code	Name
ZPPH	Zinc protoporphyrin/Heme Ratio
PB	Lead
COM9	Comment
PBMTH	Lead Method

Guidelines

Method

Hematofluorometry

Reference Range

See individual components

Specimen Type: Blood
Collection: Preferred: 7 mL blood in ROYAL BLUE (Na2 EDTA) TOP tube.
Also acceptable: LAVENDER or GREEN TOP
Unacceptable: Spun Gel separator tubes
Quantity: Requested: entire tube
Minimum: 0.5 mL wbld

Receiving Instructions: If only 1 tube is received, the specimen MUST be at Room Temperature to aliquot for ZPPH. Mix on ROCKER for 5 minutes, then aliquot 0.5 mL whole blood into a plastic vial for ZPPH. Re-stopper the ROYAL BLUE tube for PB (lead) testing.

Refrigerate both whole blood samples after aliquoting.

If the sample is <1.0 mL, it must be shared, with PB testing occurring first at Montlake, and ZPPH testing occurring last at HMC. Rack the sample in the PB rack with a shared specimen slip.

Lab Department

Chemistry, University(CHU)

Frequency

PB: Tuesdays, ZPPH: Monday-Friday

Available STAT?

Performing Location(s)

HMC	Chemistry, Special 206-520-4600 325 9th Ave, Rm # GWH-47, Seattle, WA 98104-2420
UW-MT	Chemistry, Special 206-520-4600 Clinical Lab, Room NW220, University of Washington Medical Center, 1959 NE Pacific street, Seattle, WA 98195