Methotrexate by LCMSMS
General Information
- Lab Name
- Methotrexate by LCMSMS
- Lab Code
- MTXG1
- Epic Name
- Methotrexate Level
- Description
The concentration of methotrexate in serum or plasma is determined by liquid chromatography-tandem mass spectrometry after protein precipitation. The reportable range is 0.01 to 2,000 mcmol/L with imprecision between 3 and 14 %CV.
- Components
-
Code Name MTXR Methotrexate Result MTXINF Methotrexate Information
Interpretation
- Method
Serum/plasma protein precipitation. Analysis by liquid chromatography - tandem mass spectrometry.
- Reference Range
- See individual components
Ordering & Collection
- Specimen Type
- Blood
- Collection
-
Preferred: 3 mL blood in GREEN, RED, or LAVENDER TOP tube
Acceptable: 3 mL blood in GOLD SST or LIME PST tube
- Quantity
-
Requested: 0.7 mL serum/plasma
Minimum: 0.5 mL serum/plasma
Processing
- Receiving Instructions
Remove serum or plasma from the gel separator within 4 hours of collection. Refrigerate aliquot tube.
Stability: Methotrexate in serum is stable up to 5 days when refrigerated. Avoid exposure to daylight.
- Misc Sendout
Performance
- Lab Department
- Chemistry, University(CHU)
- Frequency
- Daily, specimens must be in the lab by 09:00am for same day results
- Available STAT?
- No
- Performing Location(s)
-
UW-MT Chemistry, Special
206-520-4600Clinical Lab, Room NW220,
University of Washington Medical Center,
1959 NE Pacific street, Seattle, WA 98195
Billing & Coding
- CPT Codes
- 80204
- LOINC
- 14836-1
- Interfaced Order Code
- UOW2749