Plasmodium 18S Reflexive Sanger Sequencing

General Information

Lab Name

Lab Code

M18SEQ

Epic Name

Plasmodium 18S Reflexive Sanger Sequencing

Description

Reflex testing only. This test is a reflex test to the Plasmodium 18S rRNA quantitative RT-PCR assay (MAL18S) to identify the Plasmodium species for specimen that were non-Plasmodium falciparum Plasmodium positive. Test will be automatically ordered as indicated by MAL18S test.

This test entry is for information only and cannot be ordered. See, Plasmodium 18S rRNA quantitative RT-PCR assay [MAL18S].

This test was developed, and its performance characteristics were determined, by the University of Washington Department of Laboratory Medicine and Pathology. It is important to note that while this test has been validated for clinical use, it has not been cleared or approved by the US Food and Drug Administration (FDA). However, under the Clinical Laboratory Improvement Amendments (CLIA), the University of Washington laboratory ensures the test meets high standards for accuracy and reliability in a clinical setting.

Synonyms

MAL18S reflex, Malaria PCR, Malaria Sequencing, P. knowlesi, P. malariae, P. ovale, P. vivax, Plasmodium, Plasmodium 18S, Plasmodium identification, Plasmodium PCR, Plasmodium sequencing, Sanger

Components

Code	Name
MSQINT	Plasmodium 18S Sanger Sequence Interpretation
MSQMTH	Plasmodium 18S Sanger Sequence Method

Interpretation

Method: RNA/DNA extraction, Polymerase Chain Reaction, Sanger Sequencing

Reflexive Sanger Sequencing of amplicons produced by the Malaria Molecular Diagnostic Lab’s Plasmodium 18S rRNA/rDNA quantitative real-time reverse-transcriptase Polymerase Chain reaction (test code MAL18S). Samples that were identified as non-P. falciparum Plasmodium positive by the MAL18S test code will be prepared and sent out for CLIA Sanger Sequencing for species identification purposes.
Reference Range: See individual components
Ref. Range Notes: This test can be used for the identification of Plasmodium ovale, Plasmodium malariae, Plasmodium knowlesi, and Plasmodium vivax.

This test cannot be used to identify more than one species of Plasmodium at a time. Patients who are infected with multiple Plasmodium species will not get a clear read out and may only serve to confirm a general infection by Plasmodium.

Ordering & Collection

Specimen Type: EDTA whole blood
Quantity: Requested: 2 mL
Minimum: 0.5 mL

Processing

Receiving Instructions: This test will be ordered automatically per MAL18S results indication and will use MAL18S aliquot already in Lab.

Misc Sendout

Performance

Lab Department

Malaria Molecular Diag Lab(MMDL)

Frequency

Within 2 weeks after the reflex test is ordered.

Available STAT?

Performing Location(s)

Other	Malaria Molecular Diagnostic Laboratory Malaria Molecular Diagnostic Laboratory University of Washington Building F, Room # 870 (Call 206-685-4599 for access) 750 Republican St Seattle, WA 98109	206-685-4599 (Lab) 206-685-8212 (Office)

Billing & Coding

CPT Codes: 87798
LOINC: 47260-5