HIV1 RNA Quantitation

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General Information

Lab Name
HIV1 RNA Quantitation
Lab Code
HRTABB
Epic Ordering
HIV1 Rna Quantitation
Description

The UW Clinical Virology Laboratory in the Department of Laboratory Medicine and Pathology incorporates the FDA approved Roche cobas® HIV-1 quantitative assay for the detection and quantitation of human immunodeficiency virus type 1 in EDTA plasma using real-time PCR amplification.

The range of quantitation of HIV-1 RNA is 20 to 10,000,000 copies/mL (33 to 1.67 x 107 International Units/mL).

The Roche cobas® HIV-1 quantitative assay uses primers specific for the HIV-1 gag gene and the HIV-1 LTR region (dual target).

Synonyms
HIV PCR, HIV Viral Load
Components

Interpretation

Method

Real-time Polymerase Chain Reaction

Reference Range
See individual components
Guidelines

Ordering & Collection

Specimen Type
Plasma (EDTA)
Collection

Blood: EDTA plasma or PPT

Unacceptable: plasma from GREEN TOP (Heparin), YELLOW (ACD) or GRAY (Oxylate) and serum (No RED TOP)

Handling Instructions

Freshly drawn whole blood may be stored and/or transported for up to 24 hours at 2°C to 25°C prior to plasma preparation through centrifugation.

Quantity
requested: 2.0 mL plasma
minimum: 0.75 mL plasma

Processing

Processing

Blood: Centrifugation upon arrival, remove plasma from cells.

Plasma may be stored and/or transported:

  • 2°C to 8°C for up to 6 days
  • -18°C ± 2°C for up to 12 weeks

Outside Laboratory: Freeze at -10°C or colder and ship on dry ice. If freezer and/or dry ice is unavailable, ship immediately at 2-8°C (ice pack). Samples may be stored at 2-8°C (ice pack) for up to 48 hours of collection. Shipment time must not exceed 48 hours from collection if stored at 2-8°C. Samples stored at 2-8°C greater than 48 hours old may be rejected. Do not freeze/thaw.

Performance

LIS Dept Code
Central Lab Virology (RVIR)
Performing Location(s)
CL Virology, Renton
206-685-6656

1602 Raymond Ave SW

Renton, WA 98057-3356

206-685-6656 opt 4

Frequency
Tuesday - Saturday. Results same day.
Available STAT?
No

Billing & Coding

CPT codes
87536
LOINC
49890-7
Interfaced Order Code
UOW1094